Iron in PDB 7oqy: Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase

All present enzymatic activity of Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase:
2.7.7.6;

The structure of Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Zinc	(Zn)	8 atoms

The binding sites of Iron atom in the Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase (pdb code 7oqy). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase, PDB code: 7oqy:
Jump to Iron binding site number: 1; 2; 3;

Iron binding site 1 out of 3 in the Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe301 b:43.8 occ:1.00 FE1 D:F3S301 0.0 43.8 1.0 S3 D:F3S301 2.2 43.8 1.0 S1 D:F3S301 2.2 43.8 1.0 S2 D:F3S301 2.2 43.8 1.0 SG D:CYS183 2.3 33.8 1.0 SG D:CYS206 2.3 30.6 1.0 FE4 D:F3S301 3.0 43.8 1.0 FE3 D:F3S301 3.1 43.8 1.0 CD D:PRO184 3.5 27.8 1.0 CB D:CYS183 3.7 33.8 1.0 CA D:CYS183 3.9 33.8 1.0 CB D:CYS206 4.0 30.6 1.0 S4 D:F3S301 4.2 43.8 1.0 N D:PRO184 4.3 27.8 1.0 CG D:PRO184 4.4 27.8 1.0 C D:CYS183 4.5 33.8 1.0 SG D:CYS209 4.8 39.3 1.0 N D:CYS206 4.9 30.6 1.0 N D:GLU185 4.9 30.6 1.0 SG D:CYS203 5.0 26.4 1.0 CG D:GLU185 5.0 30.6 1.0 CA D:CYS206 5.0 30.6 1.0

Iron binding site 2 out of 3 in the Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe301 b:43.8 occ:1.00 FE3 D:F3S301 0.0 43.8 1.0 S3 D:F3S301 2.2 43.8 1.0 S4 D:F3S301 2.2 43.8 1.0 S1 D:F3S301 2.2 43.8 1.0 SG D:CYS209 2.3 39.3 1.0 FE1 D:F3S301 3.1 43.8 1.0 SG D:CYS206 3.1 30.6 1.0 FE4 D:F3S301 3.1 43.8 1.0 N D:CYS209 3.4 39.3 1.0 N D:GLU207 3.4 35.0 1.0 CB D:CYS209 3.4 39.3 1.0 N D:GLU208 3.6 37.1 1.0 CA D:GLU207 3.6 35.0 1.0 C D:GLU207 3.9 35.0 1.0 CA D:CYS209 4.0 39.3 1.0 S2 D:F3S301 4.2 43.8 1.0 C D:GLU208 4.4 37.1 1.0 C D:CYS206 4.4 30.6 1.0 CG1 D:ILE219 4.5 37.0 1.0 CD1 D:ILE219 4.5 37.0 1.0 CA D:GLU208 4.5 37.1 1.0 CB D:CYS206 4.5 30.6 1.0 N D:CYS206 4.5 30.6 1.0 SG D:CYS183 4.6 33.8 1.0 CA D:CYS206 4.7 30.6 1.0 O D:GLU207 4.8 35.0 1.0 SG D:CYS203 4.9 26.4 1.0 N D:LEU210 4.9 39.1 1.0 CB D:GLU208 4.9 37.1 1.0 C D:CYS209 4.9 39.3 1.0

Iron binding site 3 out of 3 in the Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cryo-Em Structure of the Cellular Negative Regulator TFS4 Bound to the Archaeal Rna Polymerase within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe301 b:43.8 occ:1.00 FE4 D:F3S301 0.0 43.8 1.0 S3 D:F3S301 2.2 43.8 1.0 S4 D:F3S301 2.2 43.8 1.0 S2 D:F3S301 2.2 43.8 1.0 SG D:CYS203 2.3 26.4 1.0 FE1 D:F3S301 3.0 43.8 1.0 FE3 D:F3S301 3.1 43.8 1.0 N D:LEU205 3.3 25.4 1.0 SG D:CYS206 3.4 30.6 1.0 N D:CYS206 3.4 30.6 1.0 CA D:LEU205 3.7 25.4 1.0 CB D:CYS203 3.8 26.4 1.0 N D:ILE204 3.8 24.4 1.0 C D:LEU205 4.0 25.4 1.0 CA D:CYS203 4.1 26.4 1.0 S1 D:F3S301 4.2 43.8 1.0 C D:ILE204 4.2 24.4 1.0 C D:CYS203 4.3 26.4 1.0 CB D:CYS206 4.3 30.6 1.0 CA D:CYS206 4.4 30.6 1.0 N D:GLU207 4.4 35.0 1.0 CA D:ILE204 4.6 24.4 1.0 SG D:CYS183 4.6 33.8 1.0 CD1 D:ILE219 4.8 37.0 1.0 CG1 D:ILE204 4.8 24.4 1.0 C D:CYS206 4.9 30.6 1.0 SG D:CYS209 5.0 39.3 1.0

S.Pilotto, T.Fouqueau, N.Lukoyanova, C.Sheppard, S.Lucas-Staat, L.M.Diaz-Santin, D.Matelska, D.Prangishvili, A.C.M.Cheung, F.Werner. Structural Basis of Rna Polymerase Inhibition By Viral and Host Factors Nat Commun 2021.
ISSN: ESSN 2041-1723