Iron in PDB 7p0o: Mitoneet Bound to M1 Molecule

The structure of Mitoneet Bound to M1 Molecule, PDB code: 7p0o was solved by O.Livnah, Y.Eisenberg-Domovich, H.B.Marjault, R.Nechushtai, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.11 / 1.65
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	45.152, 49.962, 58.945, 90, 90, 90
R / Rfree (%)	17.1 / 23.6

The binding sites of Iron atom in the Mitoneet Bound to M1 Molecule (pdb code 7p0o). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Mitoneet Bound to M1 Molecule, PDB code: 7p0o:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Mitoneet Bound to M1 Molecule


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Mitoneet Bound to M1 Molecule within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe200 b:27.2 occ:1.00 FE1 A:FES200 0.0 27.2 1.0 SG A:CYS74 2.2 25.9 1.0 S1 A:FES200 2.2 29.7 1.0 S2 A:FES200 2.3 27.2 1.0 SG A:CYS72 2.4 25.3 1.0 FE2 A:FES200 2.7 28.1 1.0 CB A:CYS74 3.3 26.8 1.0 CB A:CYS72 3.4 25.0 1.0 N A:CYS74 3.5 24.4 1.0 CA A:CYS72 3.8 24.9 1.0 CA A:CYS74 3.9 27.5 1.0 N A:ARG73 3.9 22.0 1.0 ND1 A:HIS87 4.1 29.9 1.0 CB A:HIS87 4.1 30.3 1.0 N A:HIS87 4.2 31.0 1.0 C A:CYS72 4.2 25.5 1.0 N A:TRP75 4.4 24.4 1.0 N A:SER77 4.4 30.6 1.0 C A:CYS74 4.4 24.1 1.0 CB A:SER77 4.5 29.6 1.0 CG A:HIS87 4.6 26.3 1.0 N A:ARG76 4.6 25.7 1.0 CA A:HIS87 4.6 34.2 1.0 C A:ARG73 4.6 25.5 1.0 SG A:CYS83 4.6 29.3 1.0 CA A:CYS83 4.9 31.1 1.0 CB A:CYS83 4.9 29.8 1.0 CA A:ARG73 4.9 23.5 1.0 CA A:SER77 4.9 31.0 1.0

Iron binding site 2 out of 4 in the Mitoneet Bound to M1 Molecule


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Mitoneet Bound to M1 Molecule within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe200 b:28.1 occ:1.00 FE2 A:FES200 0.0 28.1 1.0 ND1 A:HIS87 2.1 29.9 1.0 S2 A:FES200 2.2 27.2 1.0 SG A:CYS83 2.2 29.3 1.0 S1 A:FES200 2.3 29.7 1.0 FE1 A:FES200 2.7 27.2 1.0 CE1 A:HIS87 2.8 34.5 1.0 CG A:HIS87 3.2 26.3 1.0 CB A:CYS83 3.3 29.8 1.0 CB A:HIS87 3.8 30.3 1.0 CA A:CYS83 3.9 31.1 1.0 NE2 A:HIS87 4.0 33.3 1.0 N A:HIS87 4.1 31.0 1.0 N A:GLY85 4.1 36.7 1.0 CD2 A:HIS87 4.2 30.7 1.0 CD A:PRO100 4.2 26.0 1.0 N A:ASP84 4.3 33.1 1.0 CB A:PRO100 4.3 29.6 1.0 SG A:CYS74 4.4 25.9 1.0 CG A:PRO100 4.4 28.6 1.0 CA A:GLY85 4.4 36.5 1.0 N A:ALA86 4.5 31.6 1.0 C A:CYS83 4.5 30.7 1.0 CA A:HIS87 4.6 34.2 1.0 C A:GLY85 4.6 30.8 1.0 SG A:CYS72 4.7 25.3 1.0 CA A:CYS72 4.7 24.9 1.0 N A:PRO100 4.7 26.8 1.0 CB A:CYS72 4.8 25.0 1.0 CA A:PRO100 5.0 27.9 1.0

Iron binding site 3 out of 4 in the Mitoneet Bound to M1 Molecule


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Mitoneet Bound to M1 Molecule within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:26.1 occ:1.00 FE1 B:FES201 0.0 26.1 1.0 SG B:CYS74 2.2 25.0 1.0 S1 B:FES201 2.2 27.4 1.0 S2 B:FES201 2.2 24.4 1.0 SG B:CYS72 2.3 26.0 1.0 FE2 B:FES201 2.7 27.8 1.0 CB B:CYS74 3.3 25.0 1.0 CB B:CYS72 3.4 22.0 1.0 N B:CYS74 3.5 25.6 1.0 CA B:CYS72 3.7 23.5 1.0 CA B:CYS74 3.9 25.2 1.0 N B:ARG73 3.9 23.2 1.0 CB B:HIS87 4.0 29.0 1.0 C B:CYS72 4.2 23.1 1.0 N B:HIS87 4.2 30.5 1.0 ND1 B:HIS87 4.3 28.1 1.0 N B:TRP75 4.4 26.2 1.0 N B:SER77 4.4 28.1 1.0 C B:CYS74 4.4 22.8 1.0 CA B:HIS87 4.6 30.3 1.0 CB B:SER77 4.6 34.3 1.0 SG B:CYS83 4.6 29.4 1.0 C B:ARG73 4.6 24.6 1.0 CG B:HIS87 4.6 27.7 1.0 N B:ARG76 4.6 28.2 1.0 CA B:CYS83 4.8 29.7 1.0 CB B:CYS83 4.9 31.2 1.0 CA B:ARG73 4.9 21.1 1.0 CA B:SER77 5.0 34.0 1.0

Iron binding site 4 out of 4 in the Mitoneet Bound to M1 Molecule


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Mitoneet Bound to M1 Molecule within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:27.8 occ:1.00 FE2 B:FES201 0.0 27.8 1.0 ND1 B:HIS87 2.2 28.1 1.0 S2 B:FES201 2.2 24.4 1.0 SG B:CYS83 2.2 29.4 1.0 S1 B:FES201 2.3 27.4 1.0 FE1 B:FES201 2.7 26.1 1.0 CE1 B:HIS87 3.0 27.1 1.0 CG B:HIS87 3.2 27.7 1.0 CB B:CYS83 3.4 31.2 1.0 CB B:HIS87 3.6 29.0 1.0 CA B:CYS83 3.9 29.7 1.0 N B:HIS87 4.0 30.5 1.0 N B:GLY85 4.0 33.4 1.0 NE2 B:HIS87 4.1 27.4 1.0 CD B:PRO100 4.1 26.2 1.0 CB B:PRO100 4.2 24.5 1.0 CD2 B:HIS87 4.2 31.0 1.0 CA B:GLY85 4.3 32.7 1.0 N B:ASP84 4.4 31.1 1.0 SG B:CYS74 4.4 25.0 1.0 CA B:HIS87 4.4 30.3 1.0 CG B:PRO100 4.5 26.9 1.0 N B:ALA86 4.5 28.9 1.0 C B:CYS83 4.5 29.6 1.0 C B:GLY85 4.6 30.4 1.0 SG B:CYS72 4.6 26.0 1.0 CA B:CYS72 4.6 23.5 1.0 N B:PRO100 4.7 24.7 1.0 CB B:CYS72 4.8 22.0 1.0 CA B:PRO100 4.9 24.0 1.0

H.B.Marjault, O.Karmi, K.Zuo, D.Michaeli, Y.Eisenberg-Domovich, G.Rossetti, B.De Chassey, J.Vonderscher, I.Cabantchik, P.Carloni, R.Mittler, O.Livnah, E.Meldrum, R.Nechushtai. An Anti-Diabetic Drug Targets Neet (Cisd) Proteins Through Destabilization of Their [2FE-2S] Clusters. Commun Biol V. 5 437 2022.
ISSN: ESSN 2399-3642
PubMed: 35538231
DOI: 10.1038/S42003-022-03393-X