Iron in PDB 7tl2: Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412

The structure of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412, PDB code: 7tl2 was solved by A.M.Blee, L.E.Salay, W.J.Chazin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.02 / 1.53
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.624, 51.87, 89.479, 90, 90, 90
R / Rfree (%)	15.2 / 16.8

The binding sites of Iron atom in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412 (pdb code 7tl2). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412, PDB code: 7tl2:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe604 b:17.0 occ:1.00 FE1 A:SF4604 0.0 17.0 1.0 S4 A:SF4604 2.2 17.0 1.0 S2 A:SF4604 2.3 16.4 1.0 S3 A:SF4604 2.3 16.5 1.0 SG A:CYS336 2.3 16.5 1.0 FE3 A:SF4604 2.7 17.8 1.0 FE2 A:SF4604 2.7 17.9 1.0 FE4 A:SF4604 2.7 17.5 1.0 CB A:CYS336 3.2 16.8 1.0 S1 A:SF4604 3.9 18.4 1.0 N A:CYS336 4.1 15.5 1.0 CG A:PRO500 4.1 18.3 1.0 CA A:CYS336 4.3 15.1 1.0 CB A:PRO334 4.3 16.7 1.0 CG A:PRO334 4.4 16.5 1.0 CB A:PRO500 4.6 17.6 1.0 SG A:CYS417 4.7 19.4 1.0 SG A:CYS434 4.8 18.0 1.0 CD1 A:ILE420 4.9 17.7 1.0 SG A:CYS474 4.9 17.8 1.0

Iron binding site 2 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe604 b:17.9 occ:1.00 FE2 A:SF4604 0.0 17.9 1.0 S3 A:SF4604 2.3 16.5 1.0 S1 A:SF4604 2.3 18.4 1.0 SG A:CYS417 2.3 19.4 1.0 S4 A:SF4604 2.3 17.0 1.0 FE3 A:SF4604 2.7 17.8 1.0 FE1 A:SF4604 2.7 17.0 1.0 FE4 A:SF4604 2.7 17.5 1.0 CB A:CYS417 3.3 17.7 1.0 S2 A:SF4604 3.9 16.4 1.0 CA A:CYS417 4.0 18.2 1.0 CG A:PRO500 4.4 18.3 1.0 CM A:MPD605 4.4 23.9 1.0 CD A:PRO500 4.6 18.9 1.0 SG A:CYS474 4.7 17.8 1.0 CG2 A:THR471 4.7 22.1 1.0 SG A:CYS434 4.8 18.0 1.0 N A:PRO500 4.8 19.0 1.0 N A:CYS417 4.8 18.3 1.0 SG A:CYS336 4.9 16.5 1.0

Iron binding site 3 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe604 b:17.8 occ:1.00 FE3 A:SF4604 0.0 17.8 1.0 S2 A:SF4604 2.3 16.4 1.0 S4 A:SF4604 2.3 17.0 1.0 S1 A:SF4604 2.3 18.4 1.0 SG A:CYS474 2.3 17.8 1.0 FE4 A:SF4604 2.7 17.5 1.0 FE2 A:SF4604 2.7 17.9 1.0 FE1 A:SF4604 2.7 17.0 1.0 CB A:CYS474 3.2 14.9 1.0 S3 A:SF4604 3.8 16.5 1.0 CE2 A:PHE436 4.1 17.8 1.0 CB A:PRO334 4.1 16.7 1.0 CM A:MPD605 4.3 23.9 1.0 CD2 A:PHE436 4.4 17.6 1.0 O A:HOH773 4.4 18.6 1.0 CA A:CYS474 4.6 16.8 1.0 SG A:CYS417 4.6 19.4 1.0 SG A:CYS434 4.8 18.0 1.0 SG A:CYS336 4.8 16.5 1.0

Iron binding site 4 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF412 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe604 b:17.5 occ:1.00 FE4 A:SF4604 0.0 17.5 1.0 S1 A:SF4604 2.2 18.4 1.0 S3 A:SF4604 2.3 16.5 1.0 S2 A:SF4604 2.3 16.4 1.0 SG A:CYS434 2.3 18.0 1.0 FE3 A:SF4604 2.7 17.8 1.0 FE2 A:SF4604 2.7 17.9 1.0 FE1 A:SF4604 2.7 17.0 1.0 CB A:CYS434 3.3 16.5 1.0 S4 A:SF4604 3.9 17.0 1.0 CA A:CYS434 3.9 15.8 1.0 CD2 A:PHE436 4.1 17.6 1.0 C A:CYS434 4.7 17.2 1.0 CE2 A:PHE436 4.7 17.8 1.0 SG A:CYS336 4.7 16.5 1.0 SG A:CYS474 4.8 17.8 1.0 CB A:CYS336 4.8 16.8 1.0 CD A:PRO435 4.8 16.2 1.0 CB A:CYS474 4.8 14.9 1.0 N A:PRO435 4.9 16.8 1.0 SG A:CYS417 4.9 19.4 1.0

L.E.Salay, A.M.Blee, M.K.Raza, K.S.Gallagher, H.Chen, A.J.Dorfeuille, J.K.Barton, W.J.Chazin. Modification of the 4FE-4S Cluster Charge Transport Pathway Alters Rna Synthesis By Yeast Dna Primase. Biochemistry V. 61 1113 2022.
ISSN: ISSN 0006-2960
PubMed: 35617695
DOI: 10.1021/ACS.BIOCHEM.2C00100