Iron in PDB 7tl3: Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431

The structure of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431, PDB code: 7tl3 was solved by A.M.Blee, L.E.Salay, W.J.Chazin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.36 / 2.07
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	41.044, 51.097, 90.223, 90, 90, 90
R / Rfree (%)	23.6 / 27.7

The binding sites of Iron atom in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431 (pdb code 7tl3). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431, PDB code: 7tl3:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe601 b:36.9 occ:1.00 FE1 A:SF4601 0.0 36.9 1.0 S4 A:SF4601 2.3 30.4 1.0 S2 A:SF4601 2.3 31.7 1.0 S3 A:SF4601 2.3 31.9 1.0 SG A:CYS336 2.7 34.5 1.0 FE3 A:SF4601 2.7 34.1 1.0 FE2 A:SF4601 2.7 38.2 1.0 FE4 A:SF4601 2.7 34.9 1.0 CB A:CYS336 3.2 32.1 1.0 S1 A:SF4601 3.9 39.4 1.0 N A:CYS336 4.0 31.2 1.0 CA A:CYS336 4.2 32.0 1.0 CB A:PRO334 4.3 37.1 1.0 CG A:PRO500 4.3 38.9 1.0 CB A:PRO500 4.5 39.2 1.0 CG A:PRO334 4.6 38.5 1.0 CD1 A:ILE420 5.0 38.2 1.0

Iron binding site 2 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe601 b:38.2 occ:1.00 FE2 A:SF4601 0.0 38.2 1.0 S3 A:SF4601 2.3 31.9 1.0 S1 A:SF4601 2.3 39.4 1.0 S4 A:SF4601 2.3 30.4 1.0 SG A:CYS417 2.6 39.1 1.0 FE1 A:SF4601 2.7 36.9 1.0 FE4 A:SF4601 2.7 34.9 1.0 FE3 A:SF4601 2.7 34.1 1.0 CB A:CYS417 3.2 41.0 1.0 S2 A:SF4601 3.9 31.7 1.0 CA A:CYS417 3.9 39.5 1.0 CG A:PRO500 4.3 38.9 1.0 N A:CYS417 4.7 38.1 1.0 CD A:PRO500 4.7 39.2 1.0 N A:PRO500 4.9 39.0 1.0 CG2 A:THR471 4.9 39.5 1.0

Iron binding site 3 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe601 b:34.1 occ:1.00 FE3 A:SF4601 0.0 34.1 1.0 S2 A:SF4601 2.3 31.7 1.0 S4 A:SF4601 2.3 30.4 1.0 S1 A:SF4601 2.3 39.4 1.0 FE1 A:SF4601 2.7 36.9 1.0 FE2 A:SF4601 2.7 38.2 1.0 FE4 A:SF4601 2.7 34.9 1.0 SG A:CYS474 2.7 34.3 1.0 CB A:CYS474 3.2 34.4 1.0 S3 A:SF4601 3.9 31.9 1.0 CB A:PRO334 4.3 37.1 1.0 CE2 A:PHE436 4.4 40.2 1.0 CD2 A:PHE436 4.6 39.8 1.0 CA A:CYS474 4.7 34.3 1.0 CM A:MPD602 4.7 47.1 1.0 O A:HOH714 4.8 35.5 1.0 SG A:CYS417 4.8 39.1 1.0

Iron binding site 4 out of 4 in the Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure of Yeast P58C Multi-Tyrosine Mutant 5YF431 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe601 b:34.9 occ:1.00 FE4 A:SF4601 0.0 34.9 1.0 S3 A:SF4601 2.3 31.9 1.0 S1 A:SF4601 2.3 39.4 1.0 S2 A:SF4601 2.3 31.7 1.0 FE2 A:SF4601 2.7 38.2 1.0 FE1 A:SF4601 2.7 36.9 1.0 FE3 A:SF4601 2.7 34.1 1.0 SG A:CYS434 2.8 39.4 1.0 CB A:CYS434 3.3 37.4 1.0 S4 A:SF4601 3.9 30.4 1.0 CA A:CYS434 4.0 34.8 1.0 CD2 A:PHE436 4.2 39.8 1.0 CE2 A:PHE436 4.8 40.2 1.0 C A:CYS434 4.8 33.8 1.0 CD A:PRO435 4.9 33.2 1.0 CB A:CYS474 4.9 34.4 1.0 CB A:CYS336 4.9 32.1 1.0 O A:TYR470 4.9 40.0 1.0 N A:PRO435 5.0 32.5 1.0

L.E.Salay, A.M.Blee, M.K.Raza, K.S.Gallagher, H.Chen, A.J.Dorfeuille, J.K.Barton, W.J.Chazin. Modification of the 4FE-4S Cluster Charge Transport Pathway Alters Rna Synthesis By Yeast Dna Primase. Biochemistry V. 61 1113 2022.
ISSN: ISSN 0006-2960
PubMed: 35617695
DOI: 10.1021/ACS.BIOCHEM.2C00100