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Iron in PDB 7xbm: Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238

Enzymatic activity of Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238

All present enzymatic activity of Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238:
1.14.15.33;

Protein crystallography data

The structure of Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238, PDB code: 7xbm was solved by G.B.Li, Y.J.Pan, S.Y.Li, X.Gao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 23.51 / 2.40
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 60.04, 108.331, 153.3, 90, 90, 90
R / Rfree (%) 18.4 / 23

Other elements in 7xbm:

The structure of Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 also contains other interesting chemical elements:

Arsenic (As) 2 atoms

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 (pdb code 7xbm). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238, PDB code: 7xbm:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in 7xbm

Go back to Iron Binding Sites List in 7xbm
Iron binding site 1 out of 2 in the Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe501

b:23.4
occ:1.00
FE A:HEM501 0.0 23.4 1.0
ND A:HEM501 2.0 28.2 1.0
NA A:HEM501 2.0 26.9 1.0
NC A:HEM501 2.1 31.8 1.0
NB A:HEM501 2.1 32.4 1.0
SG A:CYS354 2.3 19.6 1.0
C1D A:HEM501 3.0 25.8 1.0
C4D A:HEM501 3.0 24.9 1.0
C4C A:HEM501 3.0 30.1 1.0
C1A A:HEM501 3.0 28.1 1.0
O3 A:GOL505 3.0 39.0 1.0
C1C A:HEM501 3.1 32.4 1.0
C4A A:HEM501 3.1 27.5 1.0
C4B A:HEM501 3.1 27.6 1.0
C1B A:HEM501 3.1 29.3 1.0
CHD A:HEM501 3.3 27.9 1.0
CHA A:HEM501 3.4 25.1 1.0
CHC A:HEM501 3.4 29.6 1.0
CB A:CYS354 3.4 23.7 1.0
CHB A:HEM501 3.4 31.0 1.0
C3 A:GOL505 3.9 34.4 1.0
O A:ALA243 4.1 55.3 1.0
CA A:CYS354 4.2 24.7 1.0
C3D A:HEM501 4.2 24.4 1.0
C2D A:HEM501 4.2 25.8 1.0
C3C A:HEM501 4.3 36.1 1.0
C2A A:HEM501 4.3 26.4 1.0
C2C A:HEM501 4.3 36.5 1.0
C3B A:HEM501 4.3 32.5 1.0
C3A A:HEM501 4.3 26.3 1.0
C2B A:HEM501 4.3 34.5 1.0
OG1 A:THR247 4.6 39.3 1.0
CB A:ALA243 4.8 30.7 1.0
C A:ALA243 4.9 42.4 1.0
N A:GLY356 4.9 25.6 1.0
C A:CYS354 5.0 23.9 1.0

Iron binding site 2 out of 2 in 7xbm

Go back to Iron Binding Sites List in 7xbm
Iron binding site 2 out of 2 in the Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe501

b:26.1
occ:1.00
FE B:HEM501 0.0 26.1 1.0
ND B:HEM501 2.0 26.7 1.0
NA B:HEM501 2.0 24.4 1.0
NC B:HEM501 2.1 34.3 1.0
NB B:HEM501 2.1 32.2 1.0
SG B:CYS354 2.3 22.1 1.0
C1D B:HEM501 3.0 26.1 1.0
C4D B:HEM501 3.0 24.3 1.0
O1 B:GOL509 3.0 41.5 1.0
C4C B:HEM501 3.0 30.3 1.0
C1A B:HEM501 3.0 26.6 1.0
C1C B:HEM501 3.1 30.2 1.0
C4A B:HEM501 3.1 25.8 1.0
C4B B:HEM501 3.1 30.3 1.0
C1B B:HEM501 3.1 27.4 1.0
CHD B:HEM501 3.3 26.3 1.0
CHA B:HEM501 3.4 23.9 1.0
CB B:CYS354 3.4 22.7 1.0
CHC B:HEM501 3.4 27.3 1.0
CHB B:HEM501 3.4 27.2 1.0
C1 B:GOL509 3.9 40.3 1.0
O B:ALA243 4.1 48.8 1.0
CA B:CYS354 4.2 23.1 1.0
C3D B:HEM501 4.2 28.0 1.0
C2D B:HEM501 4.2 23.6 1.0
C3C B:HEM501 4.3 34.5 1.0
C2A B:HEM501 4.3 26.2 1.0
C2C B:HEM501 4.3 30.4 1.0
C3A B:HEM501 4.3 26.2 1.0
C3B B:HEM501 4.3 33.1 1.0
C2B B:HEM501 4.3 32.0 1.0
OG1 B:THR247 4.6 37.4 1.0
C B:ALA243 4.8 41.1 1.0
C B:CYS354 4.9 24.9 1.0
N B:GLY356 4.9 25.2 1.0
N B:ILE355 5.0 24.6 1.0

Reference:

Y.J.Pan, G.B.Li, X.Gao, S.Y.Li. Crystal Structure of Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 Nat Commun 2023.
ISSN: ESSN 2041-1723
Page generated: Fri Aug 9 10:45:35 2024

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