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Iron in PDB 7xbn: Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238

Enzymatic activity of Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238

All present enzymatic activity of Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238:
1.14.15.33;

Protein crystallography data

The structure of Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238, PDB code: 7xbn was solved by G.B.Li, Y.J.Pan, S.Y.Li, X.Gao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 27.23 / 2.00
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 59.823, 108.903, 152.63, 90, 90, 90
R / Rfree (%) 17.5 / 21

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 (pdb code 7xbn). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238, PDB code: 7xbn:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in 7xbn

Go back to Iron Binding Sites List in 7xbn
Iron binding site 1 out of 2 in the Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe501

b:17.1
occ:1.00
FE A:HEM501 0.0 17.1 1.0
ND A:HEM501 2.0 16.9 1.0
NA A:HEM501 2.0 16.1 1.0
NC A:HEM501 2.1 17.6 1.0
NB A:HEM501 2.1 18.5 1.0
SG A:CYS354 2.3 17.4 1.0
C1D A:HEM501 3.0 17.8 1.0
C4D A:HEM501 3.0 15.2 1.0
C4C A:HEM501 3.0 19.0 1.0
C1A A:HEM501 3.0 17.5 1.0
C4B A:HEM501 3.1 17.0 1.0
C1C A:HEM501 3.1 20.5 1.0
C4A A:HEM501 3.1 15.7 1.0
C1B A:HEM501 3.1 14.7 1.0
CB A:CYS354 3.3 14.7 1.0
CHD A:HEM501 3.4 19.9 1.0
CHA A:HEM501 3.4 17.4 1.0
CHC A:HEM501 3.4 16.9 1.0
CHB A:HEM501 3.4 17.9 1.0
C1 A:PXI502 3.9 23.1 1.0
CA A:CYS354 4.0 18.4 1.0
O A:ALA243 4.2 26.8 1.0
C3D A:HEM501 4.3 18.3 1.0
C2D A:HEM501 4.3 17.0 1.0
C3C A:HEM501 4.3 15.7 1.0
C3B A:HEM501 4.3 19.0 1.0
C2A A:HEM501 4.3 12.8 1.0
C2C A:HEM501 4.3 20.0 1.0
C3A A:HEM501 4.3 16.4 1.0
C2B A:HEM501 4.3 19.3 1.0
N A:GLY356 4.7 16.5 1.0
C A:CYS354 4.8 14.2 1.0
C A:ALA243 4.8 23.8 1.0
N A:ILE355 4.8 14.2 1.0
CB A:ALA243 4.9 16.5 1.0

Iron binding site 2 out of 2 in 7xbn

Go back to Iron Binding Sites List in 7xbn
Iron binding site 2 out of 2 in the Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Yc-17-Bound Cytochrome P450 Pikc with the Unnatural Amino Acid P-Acetyl-L-Phenylalanine Incorporated at Position 238 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe501

b:16.6
occ:1.00
FE B:HEM501 0.0 16.6 1.0
ND B:HEM501 2.0 16.7 1.0
NA B:HEM501 2.0 17.3 1.0
NC B:HEM501 2.1 19.7 1.0
NB B:HEM501 2.1 20.3 1.0
SG B:CYS354 2.3 17.9 1.0
C1D B:HEM501 3.0 17.0 1.0
C4D B:HEM501 3.0 15.1 1.0
C4C B:HEM501 3.0 18.6 1.0
C1A B:HEM501 3.0 16.3 1.0
C1C B:HEM501 3.1 19.1 1.0
C4B B:HEM501 3.1 16.3 1.0
C4A B:HEM501 3.1 17.6 1.0
C1B B:HEM501 3.1 17.6 1.0
CB B:CYS354 3.3 18.0 1.0
CHD B:HEM501 3.3 17.3 1.0
CHA B:HEM501 3.4 18.4 1.0
CHC B:HEM501 3.4 17.6 1.0
CHB B:HEM501 3.4 16.9 1.0
C1 B:PXI502 4.0 28.6 1.0
CA B:CYS354 4.1 16.3 1.0
C3D B:HEM501 4.2 17.5 1.0
C2D B:HEM501 4.2 16.5 1.0
C3C B:HEM501 4.3 21.1 1.0
C2A B:HEM501 4.3 14.8 1.0
C3B B:HEM501 4.3 17.2 1.0
C2C B:HEM501 4.3 19.6 1.0
C3A B:HEM501 4.3 17.0 1.0
C2B B:HEM501 4.3 17.0 1.0
O B:ALA243 4.4 23.9 1.0
N B:GLY356 4.7 16.6 1.0
C B:CYS354 4.7 14.2 1.0
N B:ILE355 4.8 14.2 1.0
C B:ALA243 4.8 26.5 1.0
CB B:ALA243 4.9 18.8 1.0

Reference:

Y.J.Pan, G.B.Li, X.Gao, S.Y.Li. New Mechanistic Insight of Cytochrome P450 Pikc Gained From Site-Specific Mutagenesis By Non-Coding Amino Acids Nat Commun 2023.
ISSN: ESSN 2041-1723
Page generated: Fri Aug 9 10:45:38 2024

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