Iron in PDB 8d3l: Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Enzymatic activity of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
All present enzymatic activity of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer:
3.1.12.1;
Other elements in 8d3l:
The structure of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
(pdb code 8d3l). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 8 binding sites of Iron where determined in the
Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer, PDB code: 8d3l:
Jump to Iron binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Iron binding site 1 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 1 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:54.6
occ:1.00
|
FE1
|
I:SF4301
|
0.0
|
54.6
|
1.0
|
SG
|
I:CYS190
|
1.0
|
10.1
|
1.0
|
S2
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S3
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S4
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
FE4
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE3
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE2
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
CB
|
I:CYS190
|
2.8
|
10.1
|
1.0
|
SG
|
I:CYS21
|
2.9
|
8.7
|
1.0
|
S1
|
I:SF4301
|
3.9
|
54.6
|
1.0
|
CA
|
I:CYS190
|
3.9
|
10.1
|
1.0
|
N
|
I:CYS190
|
4.2
|
10.1
|
1.0
|
CB
|
I:CYS21
|
4.3
|
8.7
|
1.0
|
SG
|
I:CYS199
|
4.5
|
7.8
|
1.0
|
SG
|
I:CYS193
|
4.5
|
10.9
|
1.0
|
CG1
|
I:VAL184
|
4.7
|
8.7
|
1.0
|
CB
|
I:CYS193
|
4.9
|
10.9
|
1.0
|
O
|
I:THR186
|
4.9
|
9.9
|
1.0
|
|
Iron binding site 2 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 2 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:54.6
occ:1.00
|
FE2
|
I:SF4301
|
0.0
|
54.6
|
1.0
|
SG
|
I:CYS190
|
2.2
|
10.1
|
1.0
|
S1
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S4
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S3
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
SG
|
I:CYS199
|
2.4
|
7.8
|
1.0
|
CB
|
I:CYS190
|
2.6
|
10.1
|
1.0
|
SG
|
I:CYS193
|
2.7
|
10.9
|
1.0
|
FE3
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE4
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE1
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
CB
|
I:CYS193
|
2.9
|
10.9
|
1.0
|
CA
|
I:CYS190
|
3.6
|
10.1
|
1.0
|
CG
|
I:PRO201
|
3.8
|
8.7
|
1.0
|
S2
|
I:SF4301
|
3.9
|
54.6
|
1.0
|
CB
|
I:CYS199
|
4.1
|
7.8
|
1.0
|
CA
|
I:CYS193
|
4.4
|
10.9
|
1.0
|
O
|
I:CYS190
|
4.4
|
10.1
|
1.0
|
C
|
I:CYS190
|
4.4
|
10.1
|
1.0
|
CA
|
I:GLN196
|
4.4
|
7.5
|
1.0
|
CD
|
I:PRO201
|
4.6
|
8.7
|
1.0
|
SG
|
I:CYS21
|
4.7
|
8.7
|
1.0
|
N
|
I:CYS190
|
4.8
|
10.1
|
1.0
|
CG
|
I:GLN196
|
4.8
|
7.5
|
1.0
|
CB
|
I:GLN196
|
4.8
|
7.5
|
1.0
|
CB
|
I:PRO201
|
4.8
|
8.7
|
1.0
|
N
|
I:GLN196
|
4.9
|
7.5
|
1.0
|
O
|
I:LEU195
|
5.0
|
5.7
|
1.0
|
|
Iron binding site 3 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 3 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:54.6
occ:1.00
|
FE3
|
I:SF4301
|
0.0
|
54.6
|
1.0
|
S4
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S1
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S2
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
SG
|
I:CYS199
|
2.6
|
7.8
|
1.0
|
FE2
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE1
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE4
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
CB
|
I:CYS199
|
3.1
|
7.8
|
1.0
|
SG
|
I:CYS190
|
3.2
|
10.1
|
1.0
|
SG
|
I:CYS21
|
3.5
|
8.7
|
1.0
|
S3
|
I:SF4301
|
3.9
|
54.6
|
1.0
|
CD
|
I:ARG23
|
4.2
|
6.5
|
1.0
|
SG
|
I:CYS193
|
4.2
|
10.9
|
1.0
|
O
|
I:CYS199
|
4.3
|
7.8
|
1.0
|
CB
|
I:ARG23
|
4.3
|
6.5
|
1.0
|
CA
|
I:CYS199
|
4.4
|
7.8
|
1.0
|
C
|
I:CYS199
|
4.5
|
7.8
|
1.0
|
CB
|
I:CYS190
|
4.5
|
10.1
|
1.0
|
CG
|
I:ARG23
|
4.6
|
6.5
|
1.0
|
CG
|
I:PRO201
|
4.7
|
8.7
|
1.0
|
NE
|
I:ARG23
|
5.0
|
6.5
|
1.0
|
|
Iron binding site 4 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 4 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:54.6
occ:1.00
|
FE4
|
I:SF4301
|
0.0
|
54.6
|
1.0
|
S3
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S2
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
S1
|
I:SF4301
|
2.3
|
54.6
|
1.0
|
SG
|
I:CYS21
|
2.6
|
8.7
|
1.0
|
SG
|
I:CYS193
|
2.6
|
10.9
|
1.0
|
FE1
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE2
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
FE3
|
I:SF4301
|
2.7
|
54.6
|
1.0
|
SG
|
I:CYS190
|
2.9
|
10.1
|
1.0
|
CB
|
I:CYS21
|
3.5
|
8.7
|
1.0
|
SG
|
I:CYS199
|
3.6
|
7.8
|
1.0
|
CB
|
I:CYS193
|
3.8
|
10.9
|
1.0
|
S4
|
I:SF4301
|
3.9
|
54.6
|
1.0
|
NE2
|
I:GLN24
|
4.0
|
5.7
|
1.0
|
CB
|
I:GLN24
|
4.0
|
5.7
|
1.0
|
CG
|
I:GLN24
|
4.3
|
5.7
|
1.0
|
CB
|
I:CYS190
|
4.4
|
10.1
|
1.0
|
CB
|
I:CYS199
|
4.5
|
7.8
|
1.0
|
N
|
I:GLN24
|
4.6
|
5.7
|
1.0
|
CA
|
I:GLN24
|
4.6
|
5.7
|
1.0
|
CD
|
I:GLN24
|
4.6
|
5.7
|
1.0
|
CA
|
I:CYS193
|
4.9
|
10.9
|
1.0
|
CA
|
I:CYS21
|
5.0
|
8.7
|
1.0
|
|
Iron binding site 5 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 5 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:10.7
occ:1.00
|
FE1
|
J:SF4301
|
0.0
|
10.7
|
1.0
|
SG
|
J:CYS21
|
2.3
|
3.3
|
1.0
|
S2
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
S4
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
S3
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
SG
|
J:CYS199
|
2.4
|
4.3
|
1.0
|
FE2
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE3
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE4
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
SG
|
J:CYS190
|
3.6
|
5.5
|
1.0
|
S1
|
J:SF4301
|
3.9
|
10.7
|
1.0
|
CB
|
J:CYS199
|
4.0
|
4.3
|
1.0
|
CB
|
J:CYS21
|
4.1
|
3.3
|
1.0
|
CB
|
J:ARG23
|
4.1
|
1.8
|
1.0
|
SG
|
J:CYS193
|
4.3
|
3.4
|
1.0
|
CD
|
J:ARG23
|
4.5
|
1.8
|
1.0
|
CG
|
J:ARG23
|
4.8
|
1.8
|
1.0
|
O
|
J:CYS21
|
4.8
|
3.3
|
1.0
|
N
|
J:GLN24
|
4.8
|
2.2
|
1.0
|
CB
|
J:CYS190
|
4.9
|
5.5
|
1.0
|
CA
|
J:CYS21
|
4.9
|
3.3
|
1.0
|
CG2
|
J:VAL184
|
4.9
|
5.4
|
1.0
|
|
Iron binding site 6 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 6 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:10.7
occ:1.00
|
FE2
|
J:SF4301
|
0.0
|
10.7
|
1.0
|
S1
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
SG
|
J:CYS199
|
2.3
|
4.3
|
1.0
|
S4
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
S3
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
CB
|
J:CYS199
|
2.4
|
4.3
|
1.0
|
SG
|
J:CYS193
|
2.5
|
3.4
|
1.0
|
FE3
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE1
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE4
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
S2
|
J:SF4301
|
3.9
|
10.7
|
1.0
|
CA
|
J:CYS199
|
3.9
|
4.3
|
1.0
|
SG
|
J:CYS21
|
4.0
|
3.3
|
1.0
|
CB
|
J:CYS193
|
4.0
|
3.4
|
1.0
|
O
|
J:LEU195
|
4.1
|
2.5
|
1.0
|
SG
|
J:CYS190
|
4.2
|
5.5
|
1.0
|
C
|
J:LEU195
|
4.3
|
2.5
|
1.0
|
C
|
J:CYS199
|
4.5
|
4.3
|
1.0
|
CG
|
J:PRO201
|
4.5
|
6.9
|
1.0
|
N
|
J:GLN196
|
4.6
|
5.0
|
1.0
|
CB
|
J:LEU195
|
4.6
|
2.5
|
1.0
|
N
|
J:CYS199
|
4.6
|
4.3
|
1.0
|
CB
|
J:CYS190
|
4.6
|
5.5
|
1.0
|
CA
|
J:GLN196
|
4.7
|
5.0
|
1.0
|
CA
|
J:LEU195
|
4.9
|
2.5
|
1.0
|
|
Iron binding site 7 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 7 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 7 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:10.7
occ:1.00
|
FE3
|
J:SF4301
|
0.0
|
10.7
|
1.0
|
S1
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
SG
|
J:CYS193
|
2.3
|
3.4
|
1.0
|
S4
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
S2
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
SG
|
J:CYS190
|
2.3
|
5.5
|
1.0
|
SG
|
J:CYS21
|
2.3
|
3.3
|
1.0
|
FE4
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE2
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE1
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
CB
|
J:CYS193
|
2.9
|
3.4
|
1.0
|
CB
|
J:CYS21
|
3.4
|
3.3
|
1.0
|
CB
|
J:CYS190
|
3.4
|
5.5
|
1.0
|
S3
|
J:SF4301
|
3.9
|
10.7
|
1.0
|
CA
|
J:CYS190
|
4.0
|
5.5
|
1.0
|
SG
|
J:CYS199
|
4.2
|
4.3
|
1.0
|
CA
|
J:CYS193
|
4.4
|
3.4
|
1.0
|
CA
|
J:CYS21
|
4.8
|
3.3
|
1.0
|
CZ
|
J:PHE20
|
4.9
|
2.7
|
1.0
|
N
|
J:CYS193
|
4.9
|
3.4
|
1.0
|
N
|
J:CYS190
|
4.9
|
5.5
|
1.0
|
O
|
J:CYS190
|
5.0
|
5.5
|
1.0
|
C
|
J:CYS190
|
5.0
|
5.5
|
1.0
|
|
Iron binding site 8 out
of 8 in 8d3l
Go back to
Iron Binding Sites List in 8d3l
Iron binding site 8 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 8 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Pam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:10.7
occ:1.00
|
FE4
|
J:SF4301
|
0.0
|
10.7
|
1.0
|
S3
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
S2
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
S1
|
J:SF4301
|
2.3
|
10.7
|
1.0
|
SG
|
J:CYS190
|
2.3
|
5.5
|
1.0
|
CB
|
J:CYS190
|
2.6
|
5.5
|
1.0
|
FE3
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE1
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
FE2
|
J:SF4301
|
2.7
|
10.7
|
1.0
|
CG
|
J:PRO201
|
3.3
|
6.9
|
1.0
|
SG
|
J:CYS193
|
3.5
|
3.4
|
1.0
|
SG
|
J:CYS199
|
3.5
|
4.3
|
1.0
|
CB
|
J:PRO201
|
3.5
|
6.9
|
1.0
|
S4
|
J:SF4301
|
3.9
|
10.7
|
1.0
|
CA
|
J:CYS190
|
4.0
|
5.5
|
1.0
|
SG
|
J:CYS21
|
4.0
|
3.3
|
1.0
|
CA
|
J:PRO201
|
4.2
|
6.9
|
1.0
|
O
|
J:CYS190
|
4.3
|
5.5
|
1.0
|
CD
|
J:PRO201
|
4.4
|
6.9
|
1.0
|
CB
|
J:CYS199
|
4.4
|
4.3
|
1.0
|
N
|
J:PRO201
|
4.6
|
6.9
|
1.0
|
C
|
J:CYS190
|
4.7
|
5.5
|
1.0
|
|
Reference:
Y.Dhingra,
S.K.Suresh,
P.Juneja,
D.G.Sashital.
Pam Binding Ensures Orientational Integration During CAS4-CAS1-CAS2-Mediated Crispr Adaptation. Mol.Cell V. 82 4353 2022.
ISSN: ISSN 1097-2765
PubMed: 36272411
DOI: 10.1016/J.MOLCEL.2022.09.030
Page generated: Fri Aug 9 23:14:55 2024
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