Iron in PDB 8d3m: Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer

All present enzymatic activity of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer:
3.1.12.1;

The structure of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer also contains other interesting chemical elements:

Manganese

(Mn)

1 atom

The binding sites of Iron atom in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer (pdb code 8d3m). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer, PDB code: 8d3m:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE1 I:SF4301 0.0 54.6 1.0 SG I:CYS190 2.1 54.9 1.0 S2 I:SF4301 2.3 54.6 1.0 S3 I:SF4301 2.3 54.6 1.0 S4 I:SF4301 2.3 54.6 1.0 SG I:CYS21 2.3 50.9 1.0 FE4 I:SF4301 2.7 54.6 1.0 FE3 I:SF4301 2.7 54.6 1.0 FE2 I:SF4301 2.7 54.6 1.0 CE1 I:PHE189 3.4 57.7 1.0 SG I:CYS193 3.8 51.2 1.0 S1 I:SF4301 3.9 54.6 1.0 CD1 I:PHE189 3.9 57.7 1.0 CB I:CYS190 3.9 54.9 1.0 CB I:CYS21 4.0 50.9 1.0 SG I:CYS199 4.2 47.6 1.0 CZ I:PHE189 4.4 57.7 1.0 CG2 I:VAL184 4.7 53.0 1.0 CA I:CYS190 4.8 54.9 1.0 CB I:CYS193 4.8 51.2 1.0 N I:CYS190 5.0 54.9 1.0

Iron binding site 2 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE2 I:SF4301 0.0 54.6 1.0 SG I:CYS190 2.2 54.9 1.0 S1 I:SF4301 2.3 54.6 1.0 S4 I:SF4301 2.3 54.6 1.0 S3 I:SF4301 2.3 54.6 1.0 SG I:CYS199 2.3 47.6 1.0 SG I:CYS193 2.4 51.2 1.0 FE3 I:SF4301 2.7 54.6 1.0 FE4 I:SF4301 2.7 54.6 1.0 FE1 I:SF4301 2.7 54.6 1.0 CB I:CYS193 3.2 51.2 1.0 CB I:CYS190 3.3 54.9 1.0 S2 I:SF4301 3.9 54.6 1.0 CB I:CYS199 4.0 47.6 1.0 CA I:CYS190 4.2 54.9 1.0 SG I:CYS21 4.4 50.9 1.0 CG I:PRO201 4.4 54.0 1.0 CA I:CYS193 4.7 51.2 1.0 O I:LEU195 4.7 46.8 1.0 CA I:GLN196 4.8 47.0 1.0 C I:LEU195 4.9 46.8 1.0 N I:GLN196 4.9 47.0 1.0

Iron binding site 3 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE3 I:SF4301 0.0 54.6 1.0 S4 I:SF4301 2.3 54.6 1.0 S1 I:SF4301 2.3 54.6 1.0 S2 I:SF4301 2.3 54.6 1.0 SG I:CYS199 2.4 47.6 1.0 FE2 I:SF4301 2.7 54.6 1.0 FE1 I:SF4301 2.7 54.6 1.0 FE4 I:SF4301 2.7 54.6 1.0 CB I:CYS199 2.9 47.6 1.0 CD I:ARG23 3.4 45.1 1.0 SG I:CYS21 3.5 50.9 1.0 CB I:ARG23 3.9 45.1 1.0 S3 I:SF4301 3.9 54.6 1.0 SG I:CYS190 3.9 54.9 1.0 CG I:ARG23 4.0 45.1 1.0 SG I:CYS193 4.3 51.2 1.0 NE I:ARG23 4.3 45.1 1.0 CA I:CYS199 4.4 47.6 1.0 N I:GLN24 4.9 45.0 1.0 C I:CYS199 4.9 47.6 1.0 O I:CYS199 4.9 47.6 1.0 C I:ARG23 5.0 45.1 1.0

Iron binding site 4 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Processed Prespacer within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE4 I:SF4301 0.0 54.6 1.0 S3 I:SF4301 2.3 54.6 1.0 S2 I:SF4301 2.3 54.6 1.0 S1 I:SF4301 2.3 54.6 1.0 SG I:CYS193 2.3 51.2 1.0 SG I:CYS21 2.6 50.9 1.0 FE1 I:SF4301 2.7 54.6 1.0 FE2 I:SF4301 2.7 54.6 1.0 FE3 I:SF4301 2.7 54.6 1.0 CB I:CYS21 3.4 50.9 1.0 CB I:GLN24 3.7 45.0 1.0 SG I:CYS190 3.8 54.9 1.0 S4 I:SF4301 3.9 54.6 1.0 SG I:CYS199 3.9 47.6 1.0 CB I:CYS193 4.0 51.2 1.0 CG I:GLN24 4.1 45.0 1.0 NE2 I:GLN24 4.1 45.0 1.0 CA I:GLN24 4.1 45.0 1.0 N I:GLN24 4.2 45.0 1.0 CD I:GLN24 4.6 45.0 1.0 CA I:CYS21 4.8 50.9 1.0 CB I:CYS199 4.9 47.6 1.0 CE2 I:PHE20 5.0 55.2 1.0

Y.Dhingra, S.K.Suresh, P.Juneja, D.G.Sashital. Pam Binding Ensures Orientational Integration During CAS4-CAS1-CAS2-Mediated Crispr Adaptation. Mol.Cell V. 82 4353 2022.
ISSN: ISSN 1097-2765
PubMed: 36272411
DOI: 10.1016/J.MOLCEL.2022.09.030