Iron in PDB 8d3p: Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi)

All present enzymatic activity of Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi):
3.1.12.1;

The structure of Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi) also contains other interesting chemical elements:

Manganese

(Mn)

1 atom

The binding sites of Iron atom in the Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi) (pdb code 8d3p). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi), PDB code: 8d3p:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi) within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE1 I:SF4301 0.0 54.6 1.0 SG I:CYS190 1.6 61.0 1.0 SG I:CYS21 1.8 65.0 1.0 S2 I:SF4301 2.3 54.6 1.0 S3 I:SF4301 2.3 54.6 1.0 S4 I:SF4301 2.3 54.6 1.0 FE4 I:SF4301 2.7 54.6 1.0 FE3 I:SF4301 2.7 54.6 1.0 FE2 I:SF4301 2.7 54.6 1.0 CB I:CYS190 3.1 61.0 1.0 CB I:CYS21 3.3 65.0 1.0 S1 I:SF4301 3.9 54.6 1.0 SG I:CYS199 4.1 46.7 1.0 CA I:CYS190 4.4 61.0 1.0 CA I:CYS21 4.5 65.0 1.0 O I:CYS190 4.5 61.0 1.0 O I:CYS21 4.7 65.0 1.0 O I:THR186 4.8 59.5 1.0 OG1 I:THR186 4.8 59.5 1.0 C I:CYS190 4.9 61.0 1.0

Iron binding site 2 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi) within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE2 I:SF4301 0.0 54.6 1.0 S1 I:SF4301 2.3 54.6 1.0 S4 I:SF4301 2.3 54.6 1.0 S3 I:SF4301 2.3 54.6 1.0 SG I:CYS199 2.5 46.7 1.0 FE3 I:SF4301 2.7 54.6 1.0 FE4 I:SF4301 2.7 54.6 1.0 SG I:CYS190 2.7 61.0 1.0 FE1 I:SF4301 2.7 54.6 1.0 SG I:CYS193 3.1 58.1 1.0 CB I:CYS199 3.5 46.7 1.0 CB I:CYS193 3.6 58.1 1.0 S2 I:SF4301 3.9 54.6 1.0 O I:CYS190 3.9 61.0 1.0 SG I:CYS21 4.0 65.0 1.0 CB I:CYS190 4.3 61.0 1.0 C I:CYS190 4.7 61.0 1.0 CA I:GLN196 4.8 38.7 1.0 CA I:CYS199 4.9 46.7 1.0 CA I:CYS190 5.0 61.0 1.0

Iron binding site 3 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi) within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE3 I:SF4301 0.0 54.6 1.0 SG I:CYS199 2.0 46.7 1.0 S4 I:SF4301 2.3 54.6 1.0 S1 I:SF4301 2.3 54.6 1.0 S2 I:SF4301 2.3 54.6 1.0 FE2 I:SF4301 2.7 54.6 1.0 FE1 I:SF4301 2.7 54.6 1.0 FE4 I:SF4301 2.7 54.6 1.0 SG I:CYS21 3.1 65.0 1.0 CB I:CYS199 3.7 46.7 1.0 CB I:ARG23 3.8 51.6 1.0 S3 I:SF4301 3.9 54.6 1.0 CD I:ARG23 3.9 51.6 1.0 SG I:CYS190 4.0 61.0 1.0 CG I:ARG23 4.4 51.6 1.0 CA I:CYS199 4.6 46.7 1.0 CB I:CYS21 4.7 65.0 1.0 O I:CYS199 4.8 46.7 1.0 NH1 I:ARG23 4.9 51.6 1.0 C I:CYS199 4.9 46.7 1.0 CA I:ARG23 5.0 51.6 1.0

Iron binding site 4 out of 4 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Type I-C CAS4-CAS1-CAS2 Complex Bound to Half-Site Integration Intermediate (Hsi) within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe301 b:54.6 occ:1.00 FE4 I:SF4301 0.0 54.6 1.0 S3 I:SF4301 2.3 54.6 1.0 S2 I:SF4301 2.3 54.6 1.0 S1 I:SF4301 2.3 54.6 1.0 SG I:CYS21 2.5 65.0 1.0 FE1 I:SF4301 2.7 54.6 1.0 FE2 I:SF4301 2.7 54.6 1.0 FE3 I:SF4301 2.7 54.6 1.0 CB I:CYS21 3.2 65.0 1.0 SG I:CYS190 3.2 61.0 1.0 SG I:CYS199 3.7 46.7 1.0 S4 I:SF4301 3.9 54.6 1.0 N I:GLN24 4.1 51.2 1.0 CB I:GLN24 4.2 51.2 1.0 CB I:CYS193 4.3 58.1 1.0 CA I:GLN24 4.5 51.2 1.0 CA I:CYS21 4.6 65.0 1.0 OE1 I:GLN24 4.7 51.2 1.0 CB I:ARG23 4.8 51.6 1.0 C I:ARG23 4.9 51.6 1.0 SG I:CYS193 4.9 58.1 1.0 CB I:CYS199 4.9 46.7 1.0

Y.Dhingra, S.K.Suresh, P.Juneja, D.G.Sashital. Pam Binding Ensures Orientational Integration During CAS4-CAS1-CAS2-Mediated Crispr Adaptation. Mol.Cell V. 82 4353 2022.
ISSN: ISSN 1097-2765
PubMed: 36272411
DOI: 10.1016/J.MOLCEL.2022.09.030