Iron in PDB 8d3q: Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Enzymatic activity of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
All present enzymatic activity of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer:
3.1.12.1;
Other elements in 8d3q:
The structure of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
(pdb code 8d3q). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 8 binding sites of Iron where determined in the
Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer, PDB code: 8d3q:
Jump to Iron binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Iron binding site 1 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 1 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:83.5
occ:1.00
|
FE1
|
I:SF4301
|
0.0
|
83.5
|
1.0
|
S2
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
S4
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
S3
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
SG
|
I:CYS21
|
2.3
|
80.9
|
1.0
|
SG
|
I:CYS199
|
2.7
|
81.5
|
1.0
|
FE3
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE2
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE4
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
SG
|
I:CYS193
|
3.1
|
74.8
|
1.0
|
CB
|
I:GLN24
|
3.5
|
72.8
|
1.0
|
N
|
I:GLN24
|
3.6
|
72.8
|
1.0
|
CB
|
I:CYS21
|
3.6
|
80.9
|
1.0
|
CA
|
I:GLN24
|
3.7
|
72.8
|
1.0
|
S1
|
I:SF4301
|
3.9
|
83.5
|
1.0
|
CB
|
I:CYS199
|
3.9
|
81.5
|
1.0
|
SG
|
I:CYS190
|
4.1
|
75.2
|
1.0
|
CG
|
I:GLN24
|
4.2
|
72.8
|
1.0
|
C
|
I:ARG23
|
4.4
|
77.1
|
1.0
|
CB
|
I:ARG23
|
4.6
|
77.1
|
1.0
|
CB
|
I:CYS193
|
4.9
|
74.8
|
1.0
|
NE2
|
I:GLN24
|
4.9
|
72.8
|
1.0
|
CA
|
I:ARG23
|
5.0
|
77.1
|
1.0
|
CA
|
I:CYS21
|
5.0
|
80.9
|
1.0
|
O
|
I:ARG23
|
5.0
|
77.1
|
1.0
|
CD1
|
I:LEU27
|
5.0
|
63.8
|
1.0
|
|
Iron binding site 2 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 2 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:83.5
occ:1.00
|
FE2
|
I:SF4301
|
0.0
|
83.5
|
1.0
|
SG
|
I:CYS21
|
2.2
|
80.9
|
1.0
|
S1
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
S3
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
SG
|
I:CYS190
|
2.3
|
75.2
|
1.0
|
S4
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
SG
|
I:CYS193
|
2.6
|
74.8
|
1.0
|
FE4
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE1
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE3
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
CB
|
I:CYS21
|
2.9
|
80.9
|
1.0
|
CB
|
I:CYS193
|
3.8
|
74.8
|
1.0
|
S2
|
I:SF4301
|
3.9
|
83.5
|
1.0
|
CE2
|
I:PHE20
|
4.0
|
73.8
|
1.0
|
CB
|
I:CYS190
|
4.1
|
75.2
|
1.0
|
CA
|
I:CYS21
|
4.2
|
80.9
|
1.0
|
SG
|
I:CYS199
|
4.4
|
81.5
|
1.0
|
CD2
|
I:PHE20
|
4.4
|
73.8
|
1.0
|
CA
|
I:CYS190
|
4.8
|
75.2
|
1.0
|
CB
|
I:GLN24
|
4.9
|
72.8
|
1.0
|
|
Iron binding site 3 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 3 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:83.5
occ:1.00
|
FE3
|
I:SF4301
|
0.0
|
83.5
|
1.0
|
S4
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
S2
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
S1
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
SG
|
I:CYS199
|
2.3
|
81.5
|
1.0
|
FE1
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE2
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE4
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
SG
|
I:CYS21
|
2.9
|
80.9
|
1.0
|
CG
|
I:PRO201
|
3.2
|
92.6
|
1.0
|
SG
|
I:CYS190
|
3.2
|
75.2
|
1.0
|
S3
|
I:SF4301
|
3.9
|
83.5
|
1.0
|
CB
|
I:CYS199
|
4.0
|
81.5
|
1.0
|
CB
|
I:PRO201
|
4.1
|
92.6
|
1.0
|
SG
|
I:CYS193
|
4.3
|
74.8
|
1.0
|
CD
|
I:PRO201
|
4.3
|
92.6
|
1.0
|
O
|
I:CYS199
|
4.6
|
81.5
|
1.0
|
CB
|
I:CYS21
|
4.6
|
80.9
|
1.0
|
CB
|
I:CYS190
|
4.7
|
75.2
|
1.0
|
CB
|
I:ARG23
|
4.8
|
77.1
|
1.0
|
CG1
|
I:VAL184
|
4.9
|
96.0
|
1.0
|
|
Iron binding site 4 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 4 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Fe301
b:83.5
occ:1.00
|
FE4
|
I:SF4301
|
0.0
|
83.5
|
1.0
|
SG
|
I:CYS190
|
2.2
|
75.2
|
1.0
|
S2
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
S1
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
SG
|
I:CYS193
|
2.3
|
74.8
|
1.0
|
S3
|
I:SF4301
|
2.3
|
83.5
|
1.0
|
FE2
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE1
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
FE3
|
I:SF4301
|
2.7
|
83.5
|
1.0
|
SG
|
I:CYS199
|
3.1
|
81.5
|
1.0
|
CB
|
I:CYS193
|
3.2
|
74.8
|
1.0
|
CB
|
I:CYS190
|
3.4
|
75.2
|
1.0
|
S4
|
I:SF4301
|
3.9
|
83.5
|
1.0
|
SG
|
I:CYS21
|
4.0
|
80.9
|
1.0
|
CG
|
I:PRO201
|
4.1
|
92.6
|
1.0
|
CA
|
I:GLN196
|
4.2
|
60.6
|
1.0
|
O
|
I:CYS193
|
4.4
|
74.8
|
1.0
|
CA
|
I:CYS190
|
4.5
|
75.2
|
1.0
|
CA
|
I:CYS193
|
4.5
|
74.8
|
1.0
|
N
|
I:GLN196
|
4.6
|
60.6
|
1.0
|
C
|
I:CYS193
|
4.7
|
74.8
|
1.0
|
CG
|
I:GLN196
|
4.7
|
60.6
|
1.0
|
CB
|
I:GLN196
|
4.8
|
60.6
|
1.0
|
C
|
I:LEU195
|
4.8
|
56.0
|
1.0
|
CD
|
I:PRO201
|
4.8
|
92.6
|
1.0
|
CB
|
I:CYS199
|
4.8
|
81.5
|
1.0
|
O
|
I:LEU195
|
4.8
|
56.0
|
1.0
|
|
Iron binding site 5 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 5 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:156.4
occ:1.00
|
FE1
|
J:SF4301
|
0.0
|
156.4
|
1.0
|
S3
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S4
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S2
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
SG
|
J:CYS193
|
2.3
|
149.0
|
1.0
|
SG
|
J:CYS199
|
2.3
|
123.6
|
1.0
|
CB
|
J:CYS199
|
2.4
|
123.6
|
1.0
|
FE2
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE3
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE4
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
SG
|
J:CYS190
|
3.7
|
142.7
|
1.0
|
S1
|
J:SF4301
|
3.9
|
156.4
|
1.0
|
CA
|
J:CYS199
|
3.9
|
123.6
|
1.0
|
CB
|
J:CYS193
|
4.0
|
149.0
|
1.0
|
O
|
J:LEU195
|
4.1
|
124.9
|
1.0
|
SG
|
J:CYS21
|
4.2
|
146.9
|
1.0
|
C
|
J:LEU195
|
4.2
|
124.9
|
1.0
|
CA
|
J:GLN196
|
4.3
|
111.4
|
1.0
|
N
|
J:GLN196
|
4.3
|
111.4
|
1.0
|
N
|
J:CYS199
|
4.6
|
123.6
|
1.0
|
CB
|
J:LEU195
|
4.6
|
124.9
|
1.0
|
C
|
J:CYS199
|
4.6
|
123.6
|
1.0
|
CD1
|
J:LEU27
|
4.8
|
115.5
|
1.0
|
CA
|
J:LEU195
|
4.9
|
124.9
|
1.0
|
O
|
J:CYS193
|
4.9
|
149.0
|
1.0
|
CB
|
J:GLN196
|
5.0
|
111.4
|
1.0
|
CG
|
J:PRO201
|
5.0
|
123.9
|
1.0
|
|
Iron binding site 6 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 6 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:156.4
occ:1.00
|
FE2
|
J:SF4301
|
0.0
|
156.4
|
1.0
|
SG
|
J:CYS21
|
2.3
|
146.9
|
1.0
|
S4
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S3
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S1
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
SG
|
J:CYS193
|
2.3
|
149.0
|
1.0
|
FE1
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE3
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE4
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
CB
|
J:CYS193
|
2.8
|
149.0
|
1.0
|
CB
|
J:CYS21
|
3.4
|
146.9
|
1.0
|
S2
|
J:SF4301
|
3.9
|
156.4
|
1.0
|
OE1
|
J:GLN24
|
4.0
|
129.6
|
1.0
|
SG
|
J:CYS199
|
4.2
|
123.6
|
1.0
|
CA
|
J:CYS193
|
4.3
|
149.0
|
1.0
|
SG
|
J:CYS190
|
4.3
|
142.7
|
1.0
|
CD
|
J:GLN24
|
4.7
|
129.6
|
1.0
|
CA
|
J:CYS21
|
4.8
|
146.9
|
1.0
|
N
|
J:CYS193
|
4.8
|
149.0
|
1.0
|
CB
|
J:GLN24
|
4.8
|
129.6
|
1.0
|
CA
|
J:CYS190
|
4.9
|
142.7
|
1.0
|
CB
|
J:CYS199
|
4.9
|
123.6
|
1.0
|
CE2
|
J:PHE20
|
5.0
|
156.3
|
1.0
|
CE1
|
J:PHE189
|
5.0
|
139.4
|
1.0
|
|
Iron binding site 7 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 7 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 7 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:156.4
occ:1.00
|
FE3
|
J:SF4301
|
0.0
|
156.4
|
1.0
|
SG
|
J:CYS199
|
2.3
|
123.6
|
1.0
|
S2
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S4
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S1
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
SG
|
J:CYS21
|
2.3
|
146.9
|
1.0
|
FE2
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE1
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE4
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
CB
|
J:CYS199
|
3.7
|
123.6
|
1.0
|
S3
|
J:SF4301
|
3.9
|
156.4
|
1.0
|
CB
|
J:CYS21
|
4.1
|
146.9
|
1.0
|
CB
|
J:ARG23
|
4.2
|
120.4
|
1.0
|
SG
|
J:CYS193
|
4.2
|
149.0
|
1.0
|
N
|
J:GLN24
|
4.5
|
129.6
|
1.0
|
CD1
|
J:LEU27
|
4.6
|
115.5
|
1.0
|
CD
|
J:ARG23
|
4.7
|
120.4
|
1.0
|
SG
|
J:CYS190
|
4.7
|
142.7
|
1.0
|
CA
|
J:CYS21
|
4.9
|
146.9
|
1.0
|
C
|
J:ARG23
|
4.9
|
120.4
|
1.0
|
CA
|
J:CYS199
|
4.9
|
123.6
|
1.0
|
CG2
|
J:VAL184
|
5.0
|
118.4
|
1.0
|
CA
|
J:ARG23
|
5.0
|
120.4
|
1.0
|
O
|
J:CYS21
|
5.0
|
146.9
|
1.0
|
|
Iron binding site 8 out
of 8 in 8d3q
Go back to
Iron Binding Sites List in 8d3q
Iron binding site 8 out
of 8 in the Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 8 of Type I-C CAS4-CAS1-CAS2 Complex Bound to A Pam/Nopam Prespacer within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Fe301
b:156.4
occ:1.00
|
FE4
|
J:SF4301
|
0.0
|
156.4
|
1.0
|
SG
|
J:CYS190
|
2.1
|
142.7
|
1.0
|
S2
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S1
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
S3
|
J:SF4301
|
2.3
|
156.4
|
1.0
|
FE3
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE2
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
FE1
|
J:SF4301
|
2.7
|
156.4
|
1.0
|
CB
|
J:CYS190
|
3.2
|
142.7
|
1.0
|
SG
|
J:CYS199
|
3.5
|
123.6
|
1.0
|
SG
|
J:CYS193
|
3.5
|
149.0
|
1.0
|
SG
|
J:CYS21
|
3.7
|
146.9
|
1.0
|
CA
|
J:CYS190
|
3.8
|
142.7
|
1.0
|
S4
|
J:SF4301
|
3.9
|
156.4
|
1.0
|
O
|
J:THR186
|
4.3
|
128.3
|
1.0
|
O
|
J:CYS190
|
4.5
|
142.7
|
1.0
|
CB
|
J:PRO201
|
4.6
|
123.9
|
1.0
|
CB
|
J:CYS199
|
4.6
|
123.6
|
1.0
|
CE1
|
J:PHE189
|
4.6
|
139.4
|
1.0
|
CG
|
J:PRO201
|
4.7
|
123.9
|
1.0
|
C
|
J:CYS190
|
4.7
|
142.7
|
1.0
|
CB
|
J:CYS193
|
4.8
|
149.0
|
1.0
|
N
|
J:CYS190
|
4.9
|
142.7
|
1.0
|
CD1
|
J:PHE189
|
5.0
|
139.4
|
1.0
|
|
Reference:
Y.Dhingra,
S.K.Suresh,
P.Juneja,
D.G.Sashital.
Pam Binding Ensures Orientational Integration During CAS4-CAS1-CAS2-Mediated Crispr Adaptation. Mol.Cell V. 82 4353 2022.
ISSN: ISSN 1097-2765
PubMed: 36272411
DOI: 10.1016/J.MOLCEL.2022.09.030
Page generated: Fri Aug 9 23:15:06 2024
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