Iron in PDB 8en7: Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera.

The binding sites of Iron atom in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera. (pdb code 8en7). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 6 binding sites of Iron where determined in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera., PDB code: 8en7:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6;

Iron binding site 1 out of 6 in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera. within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:30.0 occ:0.16 NE2 A:HIS173 2.7 18.8 1.0 NE2 B:HIS173 2.8 18.8 1.0 NE2 H:HIS173 2.9 18.8 1.0 NE2 C:HIS173 2.9 18.8 1.0 CE1 A:HIS173 3.3 18.6 1.0 CE1 B:HIS173 3.4 18.6 1.0 CE1 H:HIS173 3.5 18.6 1.0 CE1 C:HIS173 3.5 18.6 1.0 CD2 A:HIS173 3.9 19.2 1.0 CD2 A:LEU169 3.9 18.8 1.0 CD2 H:LEU169 4.0 18.8 1.0 CD2 B:HIS173 4.0 19.2 1.0 CD2 H:HIS173 4.1 19.2 1.0 CD2 B:LEU169 4.1 18.8 1.0 CD2 C:HIS173 4.2 19.2 1.0 CD2 C:LEU169 4.2 18.8 1.0 ND1 A:HIS173 4.5 17.4 1.0 ND1 B:HIS173 4.6 17.4 1.0 ND1 H:HIS173 4.7 17.4 1.0 ND1 C:HIS173 4.7 17.4 1.0 CD1 A:LEU169 4.8 20.2 1.0 CG A:HIS173 4.9 19.6 1.0 CG A:LEU169 4.9 17.5 1.0 CD1 H:LEU169 4.9 20.2 1.0 CD1 B:LEU169 4.9 20.2 1.0 CG B:HIS173 5.0 19.6 1.0

Iron binding site 2 out of 6 in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera. within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe201 b:30.0 occ:0.16 NE2 D:HIS173 2.7 18.8 1.0 NE2 E:HIS173 2.7 18.8 1.0 NE2 M:HIS173 2.9 18.8 1.0 NE2 N:HIS173 2.9 18.8 1.0 CE1 D:HIS173 3.3 18.6 1.0 CE1 E:HIS173 3.3 18.6 1.0 CE1 M:HIS173 3.5 18.6 1.0 CE1 N:HIS173 3.5 18.6 1.0 CD2 D:HIS173 3.9 19.2 1.0 CD2 D:LEU169 3.9 18.8 1.0 CD2 E:HIS173 3.9 19.2 1.0 CD2 M:LEU169 4.0 18.8 1.0 CD2 E:LEU169 4.1 18.8 1.0 CD2 M:HIS173 4.1 19.2 1.0 CD2 N:HIS173 4.2 19.2 1.0 CD2 N:LEU169 4.2 18.8 1.0 ND1 D:HIS173 4.5 17.4 1.0 ND1 E:HIS173 4.6 17.4 1.0 ND1 M:HIS173 4.7 17.4 1.0 ND1 N:HIS173 4.7 17.4 1.0 CD1 D:LEU169 4.8 20.2 1.0 CG D:HIS173 4.9 19.6 1.0 CG E:HIS173 4.9 19.6 1.0 CG D:LEU169 4.9 17.5 1.0 CD1 M:LEU169 4.9 20.2 1.0 CD1 E:LEU169 4.9 20.2 1.0

Iron binding site 3 out of 6 in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera. within 5.0Å range: probe atom residue distance (Å) B Occ F:Fe201 b:30.0 occ:0.16 NE2 X:HIS173 2.7 18.8 1.0 NE2 V:HIS173 2.7 18.8 1.0 NE2 F:HIS173 2.9 18.8 1.0 NE2 G:HIS173 2.9 18.8 1.0 CE1 X:HIS173 3.3 18.6 1.0 CE1 V:HIS173 3.3 18.6 1.0 CE1 F:HIS173 3.5 18.6 1.0 CE1 G:HIS173 3.5 18.6 1.0 CD2 X:HIS173 3.9 19.2 1.0 CD2 X:LEU169 3.9 18.8 1.0 CD2 V:HIS173 3.9 19.2 1.0 CD2 F:LEU169 4.0 18.8 1.0 CD2 V:LEU169 4.1 18.8 1.0 CD2 F:HIS173 4.1 19.2 1.0 CD2 G:HIS173 4.2 19.2 1.0 CD2 G:LEU169 4.2 18.8 1.0 ND1 X:HIS173 4.6 17.4 1.0 ND1 V:HIS173 4.6 17.4 1.0 ND1 F:HIS173 4.7 17.4 1.0 ND1 G:HIS173 4.7 17.4 1.0 CD1 X:LEU169 4.8 20.2 1.0 CG X:HIS173 4.9 19.6 1.0 CG V:HIS173 4.9 19.6 1.0 CG X:LEU169 4.9 17.5 1.0 CD1 V:LEU169 4.9 20.2 1.0 CD1 F:LEU169 4.9 20.2 1.0

Iron binding site 4 out of 6 in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera. within 5.0Å range: probe atom residue distance (Å) B Occ I:Fe201 b:30.0 occ:0.16 NE2 I:HIS173 2.7 18.8 1.0 NE2 K:HIS173 2.7 18.8 1.0 NE2 W:HIS173 2.9 18.8 1.0 NE2 O:HIS173 2.9 18.8 1.0 CE1 I:HIS173 3.3 18.6 1.0 CE1 K:HIS173 3.3 18.6 1.0 CE1 W:HIS173 3.5 18.6 1.0 CE1 O:HIS173 3.5 18.6 1.0 CD2 I:HIS173 3.9 19.2 1.0 CD2 I:LEU169 3.9 18.8 1.0 CD2 K:HIS173 3.9 19.2 1.0 CD2 W:LEU169 4.0 18.8 1.0 CD2 K:LEU169 4.1 18.8 1.0 CD2 W:HIS173 4.1 19.2 1.0 CD2 O:HIS173 4.2 19.2 1.0 CD2 O:LEU169 4.2 18.8 1.0 ND1 I:HIS173 4.5 17.4 1.0 ND1 K:HIS173 4.6 17.4 1.0 ND1 W:HIS173 4.7 17.4 1.0 ND1 O:HIS173 4.7 17.4 1.0 CD1 I:LEU169 4.8 20.2 1.0 CG I:HIS173 4.9 19.6 1.0 CG K:HIS173 4.9 19.6 1.0 CG I:LEU169 4.9 17.5 1.0 CD1 W:LEU169 4.9 20.2 1.0 CD1 K:LEU169 4.9 20.2 1.0

Iron binding site 5 out of 6 in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera. within 5.0Å range: probe atom residue distance (Å) B Occ J:Fe201 b:30.0 occ:0.16 NE2 P:HIS173 2.7 18.8 1.0 NE2 J:HIS173 2.7 18.8 1.0 NE2 Q:HIS173 2.9 18.8 1.0 NE2 L:HIS173 2.9 18.8 1.0 CE1 P:HIS173 3.3 18.6 1.0 CE1 J:HIS173 3.3 18.6 1.0 CE1 Q:HIS173 3.5 18.6 1.0 CE1 L:HIS173 3.5 18.6 1.0 CD2 P:HIS173 3.9 19.2 1.0 CD2 P:LEU169 3.9 18.8 1.0 CD2 J:HIS173 3.9 19.2 1.0 CD2 Q:LEU169 4.0 18.8 1.0 CD2 J:LEU169 4.1 18.8 1.0 CD2 Q:HIS173 4.1 19.2 1.0 CD2 L:HIS173 4.2 19.2 1.0 CD2 L:LEU169 4.2 18.8 1.0 ND1 P:HIS173 4.5 17.4 1.0 ND1 J:HIS173 4.6 17.4 1.0 ND1 Q:HIS173 4.7 17.4 1.0 ND1 L:HIS173 4.7 17.4 1.0 CD1 P:LEU169 4.8 20.2 1.0 CG P:HIS173 4.9 19.6 1.0 CG J:HIS173 4.9 19.6 1.0 CG P:LEU169 4.9 17.5 1.0 CD1 J:LEU169 4.9 20.2 1.0 CD1 Q:LEU169 4.9 20.2 1.0

Iron binding site 6 out of 6 in the Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera.


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of Mouse Apoferritin Heavy Chain Without Zinc Determined Using Single- Particle Cryo-Em with Apollo Camera. within 5.0Å range: probe atom residue distance (Å) B Occ R:Fe201 b:30.0 occ:0.16 NE2 T:HIS173 2.7 18.8 1.0 NE2 U:HIS173 2.7 18.8 1.0 NE2 S:HIS173 2.9 18.8 1.0 NE2 R:HIS173 2.9 18.8 1.0 CE1 T:HIS173 3.3 18.6 1.0 CE1 U:HIS173 3.3 18.6 1.0 CE1 S:HIS173 3.5 18.6 1.0 CE1 R:HIS173 3.5 18.6 1.0 CD2 T:HIS173 3.9 19.2 1.0 CD2 T:LEU169 3.9 18.8 1.0 CD2 U:HIS173 3.9 19.2 1.0 CD2 S:LEU169 4.0 18.8 1.0 CD2 U:LEU169 4.1 18.8 1.0 CD2 S:HIS173 4.1 19.2 1.0 CD2 R:HIS173 4.2 19.2 1.0 CD2 R:LEU169 4.2 18.8 1.0 ND1 T:HIS173 4.6 17.4 1.0 ND1 U:HIS173 4.6 17.4 1.0 ND1 S:HIS173 4.7 17.4 1.0 ND1 R:HIS173 4.7 17.4 1.0 CD1 T:LEU169 4.8 20.2 1.0 CG T:HIS173 4.9 19.6 1.0 CG U:HIS173 4.9 19.6 1.0 CG T:LEU169 4.9 17.5 1.0 CD1 U:LEU169 4.9 20.2 1.0 CD1 S:LEU169 4.9 20.2 1.0

R.Peng, X.Fu, J.H.Mendez, P.S.Randolph, B.E.Bammes, S.M.Stagg. Characterizing the Resolution and Throughput of the Apollo Direct Electron Detector J Struct Biol X 2022.
ISSN: ESSN 2590-1524
DOI: 10.1016/J.YJSBX.2022.100080