Iron in PDB 9h1m: Recombinant Ferric Horseradish Peroxidase C1A

All present enzymatic activity of Recombinant Ferric Horseradish Peroxidase C1A:
1.11.1.7;

The structure of Recombinant Ferric Horseradish Peroxidase C1A, PDB code: 9h1m was solved by M.L.Nesa, S.K.Mandal, C.Toelzer, D.Humer, P.C.E.Moody, I.Berger, O.Spadiut, E.L.Raven, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.20 / 1.63
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.157, 67.061, 117.178, 90, 90, 90
R / Rfree (%)	17 / 18.9

The structure of Recombinant Ferric Horseradish Peroxidase C1A also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Iron atom in the Recombinant Ferric Horseradish Peroxidase C1A (pdb code 9h1m). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the Recombinant Ferric Horseradish Peroxidase C1A, PDB code: 9h1m:

Iron binding site 1 out of 1 in the Recombinant Ferric Horseradish Peroxidase C1A


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Recombinant Ferric Horseradish Peroxidase C1A within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe401 b:19.4 occ:1.00 FE A:HEM401 0.0 19.4 1.0 NC A:HEM401 2.0 20.7 1.0 ND A:HEM401 2.0 19.0 1.0 NE2 A:HIS170 2.0 19.4 1.0 NB A:HEM401 2.1 17.6 1.0 NA A:HEM401 2.1 19.8 1.0 C4C A:HEM401 3.0 20.3 1.0 C1C A:HEM401 3.0 20.3 1.0 CE1 A:HIS170 3.0 25.5 1.0 C1D A:HEM401 3.0 18.9 1.0 C4D A:HEM401 3.0 19.5 1.0 CD2 A:HIS170 3.0 21.2 1.0 C4B A:HEM401 3.1 20.7 1.0 C1B A:HEM401 3.1 19.1 1.0 C1A A:HEM401 3.1 19.0 1.0 C4A A:HEM401 3.1 19.3 1.0 CHD A:HEM401 3.4 19.8 1.0 CHC A:HEM401 3.4 20.8 1.0 CHA A:HEM401 3.4 20.4 1.0 CHB A:HEM401 3.4 21.2 1.0 O1 A:EDO409 4.0 25.3 1.0 ND1 A:HIS170 4.1 24.0 1.0 CG A:HIS170 4.2 21.2 1.0 C2D A:HEM401 4.2 19.6 1.0 C3C A:HEM401 4.2 20.0 1.0 C3D A:HEM401 4.2 19.8 1.0 C2C A:HEM401 4.2 20.8 1.0 C2B A:HEM401 4.2 20.6 1.0 C3B A:HEM401 4.3 20.5 1.0 C3A A:HEM401 4.3 19.0 1.0 C2A A:HEM401 4.3 19.6 1.0 CE2 A:PHE41 4.4 23.4 1.0 NE A:ARG38 4.5 22.0 1.0 CZ A:PHE221 4.8 23.3 1.0 C1 A:EDO409 4.8 29.0 1.0 CD A:ARG38 4.8 21.4 1.0 CD2 A:PHE41 4.8 24.0 1.0 CG A:ARG38 5.0 21.2 1.0

M.L.Nesa, S.K.Mandal, C.Toelzer, D.Humer, P.C.E.Moody, I.Berger, O.Spadiut, E.L.Raven. Crystal Structure of Ferric Recombinant Horseradish Peroxidase. J.Biol.Inorg.Chem. 2025.
ISSN: ESSN 1432-1327
PubMed: 40053124
DOI: 10.1007/S00775-025-02103-2