Iron in PDB 1cjx: Crystal Structure of Pseudomonas Fluorescens Hppd

All present enzymatic activity of Crystal Structure of Pseudomonas Fluorescens Hppd:
1.13.11.27;

The structure of Crystal Structure of Pseudomonas Fluorescens Hppd, PDB code: 1cjx was solved by L.Serre, A.Sailland, D.Sy, P.Boudec, A.Rolland, E.Pebay-Peroulla, C.Cohen-Addad, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	79.590, 142.750, 159.440, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 27.6

The structure of Crystal Structure of Pseudomonas Fluorescens Hppd also contains other interesting chemical elements:

Mercury

(Hg)

4 atoms

The binding sites of Iron atom in the Crystal Structure of Pseudomonas Fluorescens Hppd (pdb code 1cjx). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystal Structure of Pseudomonas Fluorescens Hppd, PDB code: 1cjx:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Crystal Structure of Pseudomonas Fluorescens Hppd


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Pseudomonas Fluorescens Hppd within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe629 b:12.7 occ:1.00 OE1 A:GLU322 2.0 14.9 1.0 NE2 A:HIS240 2.1 14.7 1.0 OXT A:ACT631 2.2 15.9 1.0 NE2 A:HIS161 2.2 14.5 1.0 O A:ACT631 2.6 20.8 1.0 C A:ACT631 2.7 21.4 1.0 CE1 A:HIS240 2.9 13.3 1.0 CD A:GLU322 3.0 18.7 1.0 CE1 A:HIS161 3.1 15.9 1.0 CD2 A:HIS240 3.2 13.7 1.0 CD2 A:HIS161 3.3 12.9 1.0 OE2 A:GLU322 3.4 15.0 1.0 CE1 A:PHE311 4.1 23.3 1.0 ND1 A:HIS240 4.1 11.2 1.0 NE2 A:GLN309 4.1 11.6 1.0 CH3 A:ACT631 4.2 18.1 1.0 ND1 A:HIS161 4.2 20.9 1.0 CG A:HIS240 4.2 14.5 1.0 CG A:GLU322 4.3 18.2 1.0 CG A:HIS161 4.4 11.0 1.0 CB A:ALA242 4.4 19.9 1.0 CZ A:PHE311 4.4 18.4 1.0 CB A:GLU322 4.5 16.4 1.0 CG2 A:THR163 4.6 9.7 1.0 CE1 A:PHE332 4.7 20.4 1.0

Iron binding site 2 out of 4 in the Crystal Structure of Pseudomonas Fluorescens Hppd


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Pseudomonas Fluorescens Hppd within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe629 b:18.6 occ:1.00 OE1 B:GLU322 2.0 19.9 1.0 NE2 B:HIS240 2.1 19.5 1.0 OXT B:ACT631 2.2 20.6 1.0 NE2 B:HIS161 2.2 19.4 1.0 O B:ACT631 2.7 23.2 1.0 C B:ACT631 2.9 22.5 1.0 CE1 B:HIS240 2.9 20.4 1.0 CD B:GLU322 3.0 15.6 1.0 CE1 B:HIS161 3.1 26.6 1.0 CD2 B:HIS240 3.2 14.4 1.0 CD2 B:HIS161 3.3 14.7 1.0 OE2 B:GLU322 3.4 12.0 1.0 CE1 B:PHE311 4.0 26.8 1.0 ND1 B:HIS240 4.1 15.3 1.0 NE2 B:GLN309 4.1 24.9 1.0 CG B:HIS240 4.2 16.2 1.0 CG B:GLU322 4.3 17.3 1.0 ND1 B:HIS161 4.3 25.0 1.0 CH3 B:ACT631 4.3 20.0 1.0 CZ B:PHE311 4.3 23.4 1.0 CG B:HIS161 4.4 24.2 1.0 CB B:ALA242 4.5 22.1 1.0 CB B:GLU322 4.5 18.0 1.0 CG2 B:THR163 4.6 14.5 1.0 CE1 B:PHE332 4.7 26.0 1.0

Iron binding site 3 out of 4 in the Crystal Structure of Pseudomonas Fluorescens Hppd


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of Pseudomonas Fluorescens Hppd within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe629 b:20.8 occ:1.00 OXT C:ACT631 2.1 20.8 1.0 OE1 C:GLU322 2.1 21.6 1.0 NE2 C:HIS240 2.1 20.6 1.0 NE2 C:HIS161 2.2 21.8 1.0 O C:ACT631 2.8 24.4 1.0 C C:ACT631 2.8 24.6 1.0 CE1 C:HIS240 2.9 21.2 1.0 CD C:GLU322 3.1 26.6 1.0 CE1 C:HIS161 3.1 30.2 1.0 CD2 C:HIS161 3.3 31.1 1.0 CD2 C:HIS240 3.3 21.1 1.0 OE2 C:GLU322 3.4 20.5 1.0 NE2 C:GLN309 4.0 27.6 1.0 CE1 C:PHE311 4.1 31.8 1.0 ND1 C:HIS240 4.1 21.5 1.0 ND1 C:HIS161 4.3 33.4 1.0 CH3 C:ACT631 4.3 22.4 1.0 CG C:HIS240 4.3 17.9 1.0 CZ C:PHE311 4.4 29.6 1.0 CG C:HIS161 4.4 27.8 1.0 CG C:GLU322 4.5 23.1 1.0 CE1 C:PHE332 4.5 30.7 1.0 CB C:ALA242 4.6 19.3 1.0 CG2 C:THR163 4.7 17.4 1.0 CB C:GLU322 4.7 21.9 1.0 CD1 C:PHE332 5.0 31.3 1.0

Iron binding site 4 out of 4 in the Crystal Structure of Pseudomonas Fluorescens Hppd


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure of Pseudomonas Fluorescens Hppd within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe629 b:18.9 occ:1.00 OE1 D:GLU322 2.0 19.9 1.0 OXT D:ACT631 2.1 20.0 1.0 NE2 D:HIS240 2.1 18.8 1.0 NE2 D:HIS161 2.1 18.7 1.0 C D:ACT631 2.8 23.0 1.0 O D:ACT631 2.8 22.1 1.0 CE1 D:HIS240 3.0 17.1 1.0 CE1 D:HIS161 3.0 23.8 1.0 CD D:GLU322 3.1 23.0 1.0 CD2 D:HIS161 3.2 21.5 1.0 CD2 D:HIS240 3.2 19.2 1.0 OE2 D:GLU322 3.4 21.6 1.0 CE1 D:PHE311 4.1 25.7 1.0 NE2 D:GLN309 4.2 20.0 1.0 ND1 D:HIS240 4.2 16.2 1.0 ND1 D:HIS161 4.2 24.5 1.0 CH3 D:ACT631 4.3 21.9 1.0 CG D:HIS161 4.3 20.6 1.0 CG D:HIS240 4.3 20.3 1.0 CG D:GLU322 4.4 18.5 1.0 CZ D:PHE311 4.4 26.2 1.0 CB D:ALA242 4.4 18.8 1.0 CE1 D:PHE332 4.5 31.8 1.0 CB D:GLU322 4.6 17.5 1.0 CG2 D:THR163 4.7 17.6 1.0

L.Serre, A.Sailland, D.Sy, P.Boudec, A.Rolland, E.Pebay-Peyroula, C.Cohen-Addad. Crystal Structure of Pseudomonas Fluorescens 4-Hydroxyphenylpyruvate Dioxygenase: An Enzyme Involved in the Tyrosine Degradation Pathway. Structure Fold.Des. V. 7 977 1999.
ISSN: ISSN 0969-2126
PubMed: 10467142
DOI: 10.1016/S0969-2126(99)80124-5