Iron in PDB 1fxa: Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120

The structure of Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120, PDB code: 1fxa was solved by W.R.Rypniewski, D.R.Breiter, M.M.Benning, G.Wesenberg, B.-H.Oh, J.L.Markley, I.Rayment, H.M.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	37.420, 38.120, 147.120, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Iron atom in the Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120 (pdb code 1fxa). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120, PDB code: 1fxa:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe100 b:16.6 occ:1.00 FE1 A:FES100 0.0 16.6 1.0 S1 A:FES100 2.2 16.9 1.0 S2 A:FES100 2.2 17.6 1.0 SG A:CYS46 2.3 18.2 1.0 SG A:CYS41 2.4 15.5 1.0 FE2 A:FES100 2.7 15.2 1.0 CB A:CYS46 3.3 24.4 1.0 CB A:CYS41 3.5 17.2 1.0 N A:CYS46 3.5 13.0 1.0 N A:CYS41 3.7 15.0 1.0 N A:ARG42 3.7 12.1 1.0 CA A:CYS46 3.7 18.3 1.0 CA A:CYS41 3.9 16.6 1.0 C A:CYS46 4.0 19.6 1.0 C A:CYS41 4.2 31.6 1.0 O A:CYS46 4.2 20.1 1.0 N A:ALA45 4.3 13.9 1.0 N A:ALA43 4.3 17.2 1.0 N A:GLY44 4.4 15.2 1.0 SG A:CYS79 4.4 15.0 1.0 C A:ALA45 4.4 15.9 1.0 CA A:ARG42 4.6 13.0 1.0 N A:SER40 4.6 12.0 1.0 N A:SER47 4.6 16.3 1.0 N A:THR48 4.7 14.9 1.0 C A:SER40 4.8 14.2 1.0 CB A:CYS49 4.8 16.0 1.0 CB A:SER40 4.9 21.4 1.0 N A:CYS49 4.9 8.0 1.0 CA A:GLY44 4.9 13.3 1.0 CA A:ALA45 4.9 11.3 1.0 CA A:SER40 5.0 12.9 1.0

Iron binding site 2 out of 4 in the Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe100 b:15.2 occ:1.00 FE2 A:FES100 0.0 15.2 1.0 S1 A:FES100 2.2 16.9 1.0 S2 A:FES100 2.2 17.6 1.0 SG A:CYS79 2.3 15.0 1.0 FE1 A:FES100 2.7 16.6 1.0 CB A:CYS49 2.9 16.0 1.0 SG A:CYS49 3.1 44.0 1.0 CB A:CYS79 3.2 13.0 1.0 N A:CYS79 4.1 11.2 1.0 N A:GLY44 4.2 15.2 1.0 CA A:CYS79 4.2 10.6 1.0 CA A:CYS49 4.2 11.0 1.0 N A:CYS49 4.2 8.0 1.0 N A:ARG42 4.3 12.1 1.0 SG A:CYS41 4.4 15.5 1.0 CA A:ARG42 4.4 13.0 1.0 CB A:LEU77 4.5 15.6 1.0 SG A:CYS46 4.5 18.2 1.0 N A:ALA43 4.5 17.2 1.0 CA A:GLY44 4.7 13.3 1.0 C A:ARG42 4.8 17.3 1.0 C A:CYS79 5.0 16.3 1.0

Iron binding site 3 out of 4 in the Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe100 b:17.8 occ:1.00 FE1 B:FES100 0.0 17.8 1.0 S1 B:FES100 2.2 12.1 1.0 S2 B:FES100 2.2 13.0 1.0 SG B:CYS41 2.3 16.5 1.0 SG B:CYS46 2.3 18.5 1.0 FE2 B:FES100 2.7 15.1 1.0 CB B:CYS46 3.5 14.5 1.0 N B:CYS46 3.6 22.8 1.0 N B:CYS41 3.6 17.1 1.0 CB B:CYS41 3.6 16.7 1.0 CA B:CYS46 3.8 18.6 1.0 N B:ARG42 3.8 9.8 1.0 CA B:CYS41 4.0 12.9 1.0 C B:CYS46 4.0 12.6 1.0 SG B:CYS79 4.1 12.2 1.0 O B:CYS46 4.2 20.6 1.0 N B:GLY44 4.3 12.1 1.0 N B:ALA45 4.3 14.9 1.0 C B:CYS41 4.3 7.6 1.0 SG B:CYS49 4.4 11.7 1.0 N B:ALA43 4.4 17.0 1.0 C B:ALA45 4.5 18.7 1.0 N B:SER40 4.6 15.4 1.0 C B:SER40 4.7 20.7 1.0 N B:SER47 4.7 11.9 1.0 CA B:ARG42 4.7 19.8 1.0 CA B:GLY44 4.8 18.1 1.0 N B:THR48 4.8 21.5 1.0 CA B:SER40 4.9 11.8 1.0 CA B:ALA45 5.0 13.7 1.0 C B:GLY44 5.0 21.0 1.0 CB B:SER40 5.0 18.6 1.0

Iron binding site 4 out of 4 in the Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystallization and Structure Determination to 2.5-Angstroms Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120 within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe100 b:15.1 occ:1.00 FE2 B:FES100 0.0 15.1 1.0 SG B:CYS79 2.1 12.2 1.0 S1 B:FES100 2.2 12.1 1.0 SG B:CYS49 2.2 11.7 1.0 S2 B:FES100 2.2 13.0 1.0 FE1 B:FES100 2.7 17.8 1.0 CB B:CYS79 3.1 10.6 1.0 CB B:CYS49 3.4 11.2 1.0 N B:CYS79 4.1 14.0 1.0 CA B:CYS79 4.1 16.6 1.0 N B:GLY44 4.2 12.1 1.0 N B:CYS49 4.4 15.1 1.0 N B:ARG42 4.4 9.8 1.0 SG B:CYS41 4.4 16.5 1.0 CA B:GLY44 4.5 18.1 1.0 CA B:CYS49 4.5 18.6 1.0 CB B:LEU77 4.6 16.4 1.0 CA B:ARG42 4.6 19.8 1.0 SG B:CYS46 4.7 18.5 1.0 N B:ALA43 4.7 17.0 1.0 C B:ARG42 5.0 20.2 1.0 C B:CYS79 5.0 36.2 1.0 O B:CYS46 5.0 20.6 1.0

W.R.Rypniewski, D.R.Breiter, M.M.Benning, G.Wesenberg, B.H.Oh, J.L.Markley, I.Rayment, H.M.Holden. Crystallization and Structure Determination to 2.5-A Resolution of the Oxidized [2FE-2S] Ferredoxin Isolated From Anabaena 7120. Biochemistry V. 30 4126 1991.
ISSN: ISSN 0006-2960
PubMed: 1902376
DOI: 10.1021/BI00231A003