Iron in PDB 1mhy: Methane Monooxygenase Hydroxylase

All present enzymatic activity of Methane Monooxygenase Hydroxylase:
1.14.13.25;

The structure of Methane Monooxygenase Hydroxylase, PDB code: 1mhy was solved by N.Elango, R.Radhakrishnan, W.A.Froland, B.J.Waller, C.A.Earhart, J.D.Lipscomb, D.H.Ohlendorf, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	5.00 / 2.00
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	264.490, 71.190, 139.440, 90.00, 90.00, 90.00
R / Rfree (%)	13.7 / n/a

The binding sites of Iron atom in the Methane Monooxygenase Hydroxylase (pdb code 1mhy). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Methane Monooxygenase Hydroxylase, PDB code: 1mhy:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Methane Monooxygenase Hydroxylase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Methane Monooxygenase Hydroxylase within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe801 b:14.1 occ:1.00 H2 D:HOH803 1.2 0.0 1.0 HD1 D:HIS147 1.3 0.0 1.0 H1 D:HOH803 1.7 0.0 1.0 O D:HOH803 1.7 11.5 1.0 OE1 D:GLU114 1.9 12.8 1.0 OE1 D:GLU144 2.0 12.5 1.0 O D:HOH804 2.2 14.8 1.0 ND1 D:HIS147 2.2 8.7 1.0 O D:HOH805 2.2 13.0 1.0 H1 D:HOH805 2.5 0.0 1.0 H1 D:HOH804 2.7 0.0 1.0 CD D:GLU114 2.9 16.4 1.0 H2 D:HOH804 3.0 0.0 1.0 CD D:GLU144 3.0 15.3 1.0 FE D:FE802 3.0 21.1 1.0 CE1 D:HIS147 3.1 9.4 1.0 H2 D:HOH805 3.2 0.0 1.0 OE2 D:GLU144 3.2 12.0 1.0 CG D:HIS147 3.3 10.1 1.0 OE2 D:GLU114 3.4 17.8 1.0 CB D:HIS147 3.7 9.6 1.0 OE1 D:GLU243 3.7 31.2 1.0 H1 D:HOH806 3.8 0.0 1.0 HD1 D:HIS246 3.9 0.0 1.0 OE2 D:GLU243 4.2 18.1 1.0 CG D:GLU114 4.2 13.2 1.0 O D:HOH806 4.2 27.8 1.0 NE2 D:HIS147 4.3 9.7 1.0 CE1 D:HIS246 4.4 17.5 1.0 CD2 D:HIS147 4.4 8.6 1.0 O D:HOH807 4.4 59.0 1.0 CD D:GLU243 4.4 28.3 1.0 H2 D:HOH807 4.4 0.0 1.0 CG D:GLU144 4.4 8.9 1.0 ND1 D:HIS246 4.5 16.9 1.0 CB D:GLU114 4.6 14.3 1.0 CA D:GLU144 4.6 9.3 1.0 OE2 D:GLU209 4.6 26.3 1.0 CA D:GLU114 4.7 12.7 1.0 CG1 D:VAL239 4.8 19.7 1.0 CB D:GLU144 4.8 9.9 1.0 O D:ASP143 4.9 13.2 1.0

Iron binding site 2 out of 2 in the Methane Monooxygenase Hydroxylase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Methane Monooxygenase Hydroxylase within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe802 b:21.1 occ:1.00 HD1 D:HIS246 1.3 0.0 1.0 H1 D:HOH804 1.7 0.0 1.0 H2 D:HOH803 1.8 0.0 1.0 OE2 D:GLU243 1.9 18.1 1.0 OE2 D:GLU209 2.0 26.3 1.0 O D:HOH803 2.0 11.5 1.0 O D:HOH804 2.2 14.8 1.0 ND1 D:HIS246 2.3 16.9 1.0 OE2 D:GLU144 2.3 12.0 1.0 H1 D:HOH803 2.4 0.0 1.0 CD D:GLU243 2.8 28.3 1.0 H2 D:HOH804 2.9 0.0 1.0 FE D:FE801 3.0 14.1 1.0 CD D:GLU209 3.0 24.9 1.0 CE1 D:HIS246 3.0 17.5 1.0 OE1 D:GLU243 3.1 31.2 1.0 CD D:GLU144 3.2 15.3 1.0 CG D:HIS246 3.4 20.4 1.0 HE22 D:GLN140 3.5 0.0 1.0 OE1 D:GLU144 3.5 12.5 1.0 CG D:GLU209 3.6 22.6 1.0 HD1 D:HIS147 3.9 0.0 1.0 CB D:HIS246 3.9 19.9 1.0 NE2 D:GLN140 3.9 14.1 1.0 O D:HOH805 4.0 13.0 1.0 OE1 D:GLU209 4.0 23.9 1.0 HE21 D:GLN140 4.2 0.0 1.0 NE2 D:HIS246 4.2 16.1 1.0 H1 D:HOH806 4.2 0.0 1.0 CG D:GLU243 4.2 28.2 1.0 CD2 D:HIS246 4.4 15.0 1.0 ND1 D:HIS147 4.5 8.7 1.0 H2 D:HOH805 4.5 0.0 1.0 H1 D:HOH805 4.6 0.0 1.0 OE1 D:GLU114 4.6 12.8 1.0 CG D:GLU144 4.6 8.9 1.0 CE1 D:HIS147 4.6 9.4 1.0 CD D:GLN140 4.7 18.8 1.0 O D:HOH806 4.8 27.8 1.0 O D:HOH807 4.8 59.0 1.0 CB D:GLU209 4.8 20.9 1.0 H2 D:HOH809 4.9 0.0 1.0 CG D:GLN140 5.0 14.0 1.0

N.Elango, R.Radhakrishnan, W.A.Froland, B.J.Wallar, C.A.Earhart, J.D.Lipscomb, D.H.Ohlendorf. Crystal Structure of the Hydroxylase Component of Methane Monooxygenase From Methylosinus Trichosporium OB3B Protein Sci. V. 6 556 1997.
ISSN: ISSN 0961-8368
PubMed: 9070438