Iron in PDB 1sp9: 4-Hydroxyphenylpyruvate Dioxygenase

All present enzymatic activity of 4-Hydroxyphenylpyruvate Dioxygenase:
1.13.11.27;

The structure of 4-Hydroxyphenylpyruvate Dioxygenase, PDB code: 1sp9 was solved by I.M.Fritze, L.Linden, J.Freigang, G.Auerbach, R.Huber, S.Steinbacher, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	16.83 / 3.00
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	95.200, 95.200, 185.700, 90.00, 90.00, 90.00
R / Rfree (%)	23.7 / 31.2

The binding sites of Iron atom in the 4-Hydroxyphenylpyruvate Dioxygenase (pdb code 1sp9). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the 4-Hydroxyphenylpyruvate Dioxygenase, PDB code: 1sp9:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the 4-Hydroxyphenylpyruvate Dioxygenase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of 4-Hydroxyphenylpyruvate Dioxygenase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe446 b:32.0 occ:1.00 NE2 A:HIS308 2.2 10.8 1.0 OE2 A:GLU394 2.4 23.8 1.0 NE2 A:HIS226 2.7 21.5 1.0 CE1 A:HIS308 3.2 11.6 1.0 CD2 A:HIS308 3.2 12.8 1.0 CD A:GLU394 3.3 21.7 1.0 OE1 A:GLU394 3.5 21.2 1.0 CD2 A:HIS226 3.6 21.2 1.0 CE1 A:HIS226 3.7 21.1 1.0 CE1 A:PHE381 4.3 18.7 1.0 ND1 A:HIS308 4.3 12.8 1.0 CG A:HIS308 4.4 13.9 1.0 CZ A:PHE381 4.4 20.1 1.0 CG2 A:VAL228 4.5 13.7 1.0 CG A:GLU394 4.7 20.2 1.0 CB A:ALA310 4.7 15.3 1.0 CG A:HIS226 4.8 21.3 1.0 ND1 A:HIS226 4.8 21.7 1.0

Iron binding site 2 out of 2 in the 4-Hydroxyphenylpyruvate Dioxygenase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of 4-Hydroxyphenylpyruvate Dioxygenase within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe446 b:24.3 occ:1.00 OE2 B:GLU394 2.3 28.9 1.0 NE2 B:HIS308 2.4 12.2 1.0 NE2 B:HIS226 2.5 22.1 1.0 CD B:GLU394 3.2 26.5 1.0 CE1 B:HIS308 3.4 14.9 1.0 CE1 B:HIS226 3.4 22.8 1.0 CD2 B:HIS308 3.5 13.8 1.0 OE1 B:GLU394 3.5 25.1 1.0 CD2 B:HIS226 3.5 22.4 1.0 CG2 B:VAL228 4.2 17.4 1.0 CZ B:PHE381 4.2 13.5 1.0 CB B:ALA310 4.5 16.2 1.0 CE1 B:PHE381 4.5 13.8 1.0 ND1 B:HIS308 4.5 15.0 1.0 ND1 B:HIS226 4.5 24.1 1.0 CG B:GLU394 4.5 24.9 1.0 CG B:HIS308 4.6 15.1 1.0 CG B:HIS226 4.6 22.8 1.0 CD1 B:PHE419 4.8 14.9 1.0 CB B:GLU394 4.9 20.9 1.0

I.M.Fritze, L.Linden, J.Freigang, G.Auerbach, R.Huber, S.Steinbacher. The Crystal Structures of Zea Mays and Arabidopsis 4-Hydroxyphenylpyruvate Dioxygenase Plant Physiol. V. 134 1388 2004.
ISSN: ISSN 0032-0889
PubMed: 15084729
DOI: 10.1104/PP.103.034082