Iron in PDB 2an2: P332G, A333S Double Mutant of the Bacillus Subtilis Nitric Oxide Synthase

All present enzymatic activity of P332G, A333S Double Mutant of the Bacillus Subtilis Nitric Oxide Synthase:
1.14.13.39;

The structure of P332G, A333S Double Mutant of the Bacillus Subtilis Nitric Oxide Synthase, PDB code: 2an2 was solved by K.Pant, B.R.Crane, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.60
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	80.603, 93.515, 63.571, 90.00, 90.00, 90.00
R / Rfree (%)	21.6 / 23.7

The binding sites of Iron atom in the P332G, A333S Double Mutant of the Bacillus Subtilis Nitric Oxide Synthase (pdb code 2an2). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the P332G, A333S Double Mutant of the Bacillus Subtilis Nitric Oxide Synthase, PDB code: 2an2:

Iron binding site 1 out of 1 in the P332G, A333S Double Mutant of the Bacillus Subtilis Nitric Oxide Synthase


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of P332G, A333S Double Mutant of the Bacillus Subtilis Nitric Oxide Synthase within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe901 b:44.1 occ:1.00 FE A:HEM901 0.0 44.1 1.0 NA A:HEM901 1.9 34.2 1.0 ND A:HEM901 2.0 41.7 1.0 SG A:CYS62 2.0 46.8 1.0 NC A:HEM901 2.1 41.0 1.0 NB A:HEM901 2.2 38.0 1.0 C4A A:HEM901 2.9 40.0 1.0 C1A A:HEM901 2.9 38.8 1.0 C4D A:HEM901 3.0 44.5 1.0 C4C A:HEM901 3.1 44.3 1.0 C1D A:HEM901 3.1 45.5 1.0 C1C A:HEM901 3.1 40.8 1.0 C1B A:HEM901 3.1 31.4 1.0 CB A:CYS62 3.2 44.2 1.0 C4B A:HEM901 3.2 39.5 1.0 CHA A:HEM901 3.2 41.5 1.0 CHB A:HEM901 3.3 36.2 1.0 CHD A:HEM901 3.5 41.2 1.0 CHC A:HEM901 3.5 39.7 1.0 NH1 A:ARG2000 3.9 46.0 1.0 CA A:CYS62 3.9 44.0 1.0 C3A A:HEM901 4.1 42.3 1.0 C2A A:HEM901 4.1 35.5 1.0 C3D A:HEM901 4.2 37.5 1.0 CZ A:ARG2000 4.3 45.3 1.0 C2D A:HEM901 4.3 39.5 1.0 C2C A:HEM901 4.4 35.9 1.0 C3B A:HEM901 4.4 31.0 1.0 C2B A:HEM901 4.4 35.0 1.0 C3C A:HEM901 4.5 37.8 1.0 NE1 A:TRP56 4.5 47.0 1.0 NE A:ARG2000 4.8 35.4 1.0 C A:CYS62 4.9 45.6 1.0 NH2 A:ARG2000 4.9 30.9 1.0 CD A:ARG2000 4.9 40.5 1.0 N A:ILE63 5.0 45.1 1.0 N A:CYS62 5.0 44.0 1.0

K.Pant, B.R.Crane. Structure of A Loose Dimer: An Intermediate in Nitric Oxide Synthase Assembly J.Mol.Biol. V. 352 932 2005.
ISSN: ISSN 0022-2836
PubMed: 16126221
DOI: 10.1016/J.JMB.2005.07.070