Iron in PDB 2at3: 1.00 A Crystal Structure of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed with Imidazole at pH 5.6

The structure of 1.00 A Crystal Structure of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed with Imidazole at pH 5.6, PDB code: 2at3 was solved by A.M.Amoia, W.R.Montfort, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.81 / 1.00
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	70.221, 42.743, 52.886, 90.00, 94.09, 90.00
R / Rfree (%)	14.4 / 16.7

The binding sites of Iron atom in the 1.00 A Crystal Structure of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed with Imidazole at pH 5.6 (pdb code 2at3). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the 1.00 A Crystal Structure of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed with Imidazole at pH 5.6, PDB code: 2at3:

Iron binding site 1 out of 1 in the 1.00 A Crystal Structure of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed with Imidazole at pH 5.6


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of 1.00 A Crystal Structure of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed with Imidazole at pH 5.6 within 5.0Å range: probe atom residue distance (Å) B Occ X:Fe185 b:7.5 occ:1.00 FE X:HEM185 0.0 7.5 1.0 NA X:HEM185 2.0 7.5 1.0 N1 X:IMD186 2.0 9.5 1.0 NC X:HEM185 2.0 8.4 1.0 NE2 X:HIS59 2.0 6.7 1.0 NB X:HEM185 2.0 7.4 1.0 ND X:HEM185 2.0 8.7 1.0 C5 X:IMD186 3.0 12.7 1.0 CE1 X:HIS59 3.0 5.8 1.0 C2 X:IMD186 3.0 10.4 1.0 CD2 X:HIS59 3.0 6.3 1.0 C1C X:HEM185 3.0 9.1 1.0 C4A X:HEM185 3.0 8.0 1.0 C1A X:HEM185 3.0 7.7 1.0 C4B X:HEM185 3.0 9.1 1.0 C1B X:HEM185 3.0 7.6 1.0 C4C X:HEM185 3.0 10.2 1.0 C1D X:HEM185 3.0 10.5 1.0 C4D X:HEM185 3.0 9.0 1.0 CHB X:HEM185 3.4 7.9 1.0 CHC X:HEM185 3.4 9.8 1.0 CHD X:HEM185 3.4 10.8 1.0 CHA X:HEM185 3.4 9.1 1.0 ND1 X:HIS59 4.1 6.1 1.0 N3 X:IMD186 4.1 10.2 1.0 C4 X:IMD186 4.1 13.0 1.0 CG X:HIS59 4.1 6.2 1.0 C2C X:HEM185 4.2 11.2 1.0 C3A X:HEM185 4.2 8.3 1.0 C3C X:HEM185 4.2 10.6 1.0 C2A X:HEM185 4.3 8.0 1.0 C3B X:HEM185 4.3 9.1 1.0 C2B X:HEM185 4.3 8.6 1.0 C3D X:HEM185 4.3 10.2 1.0 C2D X:HEM185 4.3 11.7 1.0

A.M.Amoia, W.R.Montfort. Heme Distortion in Nitrophorin 4: High Resolution Structures of Mutated Positions L123V and L133V and Heme Altered Proteins To Be Published.